Department of Applied Chemistry, College of Science, Northeast Agricultural University, Harbin 150030, PR China; College of Life Science, Northeast Agricultural University, Harbin 150030, PR China.
Department of Applied Chemistry, College of Science, Northeast Agricultural University, Harbin 150030, PR China; College of Life Science, Northeast Agricultural University, Harbin 150030, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Nov 5;222:117242. doi: 10.1016/j.saa.2019.117242. Epub 2019 Jun 7.
An effective morpholine-type naphthalimide chemsensor, N-p-chlorophenyl-4-(2-aminoethyl)morpholine-1,8-naphthalimide (CMN) has been developed as a lysosome-targeted fluorometric sensor for trivalent metal ions (Fe, Al and Cr). Upon the addition of Fe, Al or Cr ions, the probe CMN showed an evident naked-eye color changes which pale yellow solution of CMN turned deepened and it displayed turn-on fluorescence response in methanol. CMN showed a significant selective and sensitive toward Fe, Al or Cr ions, while there was no obvious behavior to other monovalent or divalent metal ions from the UV-vis and fluorescence spectrum. Based on the Job's plot analyses the 1:1 coordination mode of CMN with Fe, Al or Cr was proposed. The limit of detection (LOD) observed were 0.65, 0.69 and 0.68 μM for Fe, Al and Cr ions, respectively. The N-atom of morpholine directly involved in complex formation, CMN emitted fluorescence through inhibition of photoinduced electron transfer (PET). This probe exhibited excellent imaging ability for Fe, Aland Cr ions in living cells with low cytotoxicity. Significantly, the cellular confocal microscopic research indicated that the lysosome-targeted group of morpholine moiety was introduced which realized the capability of imaging lysosomal trivalent metal ions in living cells for the first time.
一种有效的吗啉型萘酰亚胺化学传感器，N-对氯苯基-4-(2-氨乙基)吗啉-1,8-萘酰亚胺（CMN）已被开发为用于三价金属离子（Fe、Al 和 Cr）的溶酶体靶向荧光传感器。当加入 Fe、Al 或 Cr 离子时，探针 CMN 表现出明显的肉眼可见的颜色变化，淡黄色的 CMN 溶液变为深黄色，并在甲醇中显示出开启荧光响应。CMN 对 Fe、Al 或 Cr 离子表现出显著的选择性和灵敏性，而紫外可见和荧光光谱对其他单价或二价金属离子没有明显的行为。基于 Job 图分析，提出了 CMN 与 Fe、Al 或 Cr 的 1:1 配位模式。观察到的检测限（LOD）分别为 0.65、0.69 和 0.68µM 用于 Fe、Al 和 Cr 离子。吗啉的 N 原子直接参与配合物的形成，CMN 通过抑制光诱导电子转移（PET）发出荧光。该探针在活细胞中对 Fe、Al 和 Cr 离子具有优异的成像能力，细胞毒性低。重要的是，细胞共聚焦显微镜研究表明，引入了靶向溶酶体的吗啉基团，首次实现了在活细胞中成像溶酶体三价金属离子的能力。
