Carvalho Elisa B, Gionbelli Mateus P, Rodrigues Rafael T S, Bonilha Sarah F M, Newbold Charles J, Guimarães Simone E F, Silva Walmir, Verardo Lucas L, Silva Fabyano F, Detmann Edenio, Duarte Marcio S
Department of Animal Science, Universidade Federal de Lavras, Lavras, MG, Brazil.
Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
BMC Genomics. 2019 Jun 17;20(1):501. doi: 10.1186/s12864-019-5890-z.
Feed efficiency is one of the most important parameters that affect beef production costs. The energy metabolism of skeletal muscle greatly contributes to variations in feed efficiency. However, information regarding differences in proteins involved in the energy metabolism of the skeletal muscle in beef cattle divergently identified for feed efficiency is scarce. In this study, we aimed to investigate energy metabolism of skeletal muscle of Nellore beef cattle, identified for low and high residual feed intake using a proteomics approach. We further assessed the expression of candidate microRNAs as a one of the possible mechanisms controlling the biosynthesis of the proteins involved in energy metabolism that were differentially abundant between high and low residual feed intake animals.
A greater abundance of 14-3-3 protein epsilon (P = 0.01) was observed in skeletal muscle of residual feed intake (RFI) high animals (RFI-High). Conversely, a greater abundance of Heat Shock Protein Beta 1 (P < 0.01) was observed in the skeletal muscle of RFI-Low cattle. A greater mRNA expression of YWHAE, which encodes the 14-3-3 protein epsilon, was also observed in the skeletal muscle of RFI-High animals (P = 0.01). A lower mRNA expression of HSPB1, which encodes the Heat Shock Protein Beta 1, was observed in the skeletal muscle of RFI-High animals (P = 0.01). The miR-665 was identified as a potential regulator of the 14-3-3 protein epsilon, and its expression was greater in RFI-Low animals (P < .001). A greater expression of miR-34a (P = 0.01) and miR-2899 (P < .001) was observed in the skeletal muscle of RFI-High animals, as both miRNAs were identified as potential regulators of HSPB1 expression.
Our results show that Nellore cattle divergently identified for feed efficiency by RFI present changes in the abundance of proteins involved in energy expenditure in skeletal muscle. Moreover, our data point towards that miR-665, miR34a and miR-2899 are likely involved in controlling both 14-3-3 epsilon and HSPB1 proteins identified as differentially abundant in the skeletal muscle of RFI-High and RFI-Low Nellore cattle.
饲料效率是影响牛肉生产成本的最重要参数之一。骨骼肌的能量代谢对饲料效率的差异有很大影响。然而，关于饲料效率差异较大的肉牛骨骼肌能量代谢相关蛋白质差异的信息却很少。在本研究中，我们旨在使用蛋白质组学方法研究内洛尔肉牛骨骼肌的能量代谢，这些肉牛根据剩余采食量分为高、低两组。我们进一步评估了候选微小RNA的表达，作为控制高、低剩余采食量动物之间能量代谢相关蛋白质生物合成的可能机制之一。
在剩余采食量（RFI）高的动物（RFI-高）的骨骼肌中观察到14-3-3蛋白ε的丰度更高（P = 0.01）。相反，在RFI-低的牛的骨骼肌中观察到热休克蛋白β1的丰度更高（P < 0.01）。在RFI-高的动物的骨骼肌中也观察到编码14-3-3蛋白ε的YWHAE的mRNA表达更高（P = 0.01）。在RFI-高的动物的骨骼肌中观察到编码热休克蛋白β1的HSPB1的mRNA表达较低（P = 0.01）。miR-665被确定为14-3-3蛋白ε的潜在调节因子，其在RFI-低的动物中的表达更高（P < 0.001）。在RFI-高的动物的骨骼肌中观察到miR-34a（P = 0.01）和miR-2899（P < 0.001）的表达更高，因为这两种微小RNA都被确定为HSPB1表达的潜在调节因子。
我们的结果表明，通过RFI鉴定出的饲料效率差异较大的内洛尔牛，其骨骼肌中参与能量消耗的蛋白质丰度存在变化。此外，我们的数据表明，miR-665、miR34a和miR-2899可能参与控制在RFI-高和RFI-低的内洛尔牛骨骼肌中差异丰富的14-3-3ε和HSPB1蛋白。
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