• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

毕赤酵母工程菌生产选择性免疫调节剂 Fusaruside。

Engineered Pichia pastoris production of fusaruside, a selective immunomodulator.

机构信息

College of Life Science, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, 271016, Shandong, China.

Department of Microbiology, College of Life Science, Key Laboratory for Agriculture Microbiology, Shandong Agricultural University, Taian, 271018, China.

出版信息

BMC Biotechnol. 2019 Jun 17;19(1):37. doi: 10.1186/s12896-019-0532-8.

DOI:10.1186/s12896-019-0532-8
PMID:31208387
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6580515/
Abstract

BACKGROUD

Fusaruside is an immunomodulatory fungal sphingolipid which has medical potentials for treating colitis and liver injury, but its poor natural abundance limits its further study.

RESULTS

In this study, we described a synthetic biology approach for fusaruside production by engineered Pichia pastoris that was based on polycistronic expression. Two fusaruside biosynthesis genes (Δ3(E)-sd and Δ10(E)-sd), were introduced into P. pastoris to obtain fusaruside producing strain FUS2. To further enhance the yield of fusaruside, three relevant biosynthetic genes (Δ3(E)-sd, Δ10(E)-sd and gcs) were subsequently introduced into P. pastoris to obtain FUS3. All of the biosynthetic genes were successfully co-expressed in FUS2 and FUS3. Compared to that produced by FUS2, fusaruside achieved from FUS3 were slightly increased. In addition, the culture conditions including pH, temperature and methanol concentration were optimized to improve the fusaruside production level.

CONCLUSIONS

Here a novel P. pastoris fusaruside production system was developed by introducing the biosynthetic genes linked by 2A peptide gene sequences into a polycistronic expression construct, laying a foundation for further development and application of fusaruside.

摘要

背景

Fusaruside 是一种具有免疫调节作用的真菌神经酰胺，具有治疗结肠炎和肝损伤的医学潜力，但由于其天然丰度低，限制了其进一步的研究。

结果

在本研究中，我们描述了一种基于多顺反子表达的工程毕赤酵母 Fusaruside 生产的合成生物学方法。将两个 Fusaruside 生物合成基因（Δ3（E）-sd 和 Δ10（E）-sd）引入毕赤酵母中，获得 Fusaruside 产生菌株 FUS2。为了进一步提高 Fusaruside 的产量，随后将三个相关生物合成基因（Δ3（E）-sd、Δ10（E）-sd 和 gcs）引入毕赤酵母中，获得 FUS3。所有生物合成基因均成功在 FUS2 和 FUS3 中共同表达。与 FUS2 相比，FUS3 产生的 Fusaruside 略有增加。此外，还优化了培养条件，包括 pH 值、温度和甲醇浓度，以提高 Fusaruside 的生产水平。

结论

本研究通过将生物合成基因连接到 2A 肽基因序列的多顺反子表达构建体中，引入毕赤酵母中，开发了一种新型毕赤酵母 Fusaruside 生产系统，为 Fusaruside 的进一步开发和应用奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bf3/6580515/2d476cd1a12d/12896_2019_532_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bf3/6580515/fba824565d2f/12896_2019_532_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bf3/6580515/56c1e52fa16e/12896_2019_532_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bf3/6580515/8a4c31fe8c24/12896_2019_532_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bf3/6580515/c9ff9a30f7d6/12896_2019_532_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bf3/6580515/2d476cd1a12d/12896_2019_532_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bf3/6580515/fba824565d2f/12896_2019_532_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bf3/6580515/56c1e52fa16e/12896_2019_532_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bf3/6580515/8a4c31fe8c24/12896_2019_532_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bf3/6580515/c9ff9a30f7d6/12896_2019_532_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bf3/6580515/2d476cd1a12d/12896_2019_532_Fig5_HTML.jpg

相似文献

1
Engineered Pichia pastoris production of fusaruside, a selective immunomodulator.毕赤酵母工程菌生产选择性免疫调节剂 Fusaruside。
BMC Biotechnol. 2019 Jun 17;19(1):37. doi: 10.1186/s12896-019-0532-8.
2
Δ10(E)-Sphingolipid Desaturase Involved in Fusaruside Mycosynthesis and Stress Adaptation in Fusarium graminearum.参与禾谷镰刀菌中镰刀菌素生物合成和胁迫适应的Δ10（E）-鞘脂去饱和酶
Sci Rep. 2015 May 21;5:10486. doi: 10.1038/srep10486.
3
Toward the construction of a technology platform for chemicals production from methanol: D-lactic acid production from methanol by an engineered yeast Pichia pastoris.朝着构建甲醇制化学品技术平台的方向前进：通过工程化酵母巴斯德毕赤酵母从甲醇生产 D-乳酸。
World J Microbiol Biotechnol. 2019 Feb 4;35(2):37. doi: 10.1007/s11274-019-2610-4.
4
Methylotrophic yeast Pichia pastoris as a chassis organism for polyketide synthesis via the full citrinin biosynthetic pathway.甲基营养型酵母巴斯德毕赤酵母作为通过完整桔霉素生物合成途径进行聚酮化合物合成的底盘生物。
J Biotechnol. 2017 Jan 20;242:64-72. doi: 10.1016/j.jbiotec.2016.11.031. Epub 2016 Nov 29.
5
Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1.通过转录因子 Mxr1 的合成正反馈回路来提高巴斯德毕赤酵母 AOX1 启动子的效率。
BMC Biotechnol. 2018 Dec 27;18(1):81. doi: 10.1186/s12896-018-0492-4.
6
Characterization and application of a putative transcription factor (SUT2) in Pichia pastoris.毕赤酵母假定转录因子（SUT2）的特性与应用。
Mol Genet Genomics. 2020 Sep;295(5):1295-1304. doi: 10.1007/s00438-020-01697-3. Epub 2020 Jun 21.
7
Metabolic engineering of Pichia pastoris for the production of dammarenediol-II.用于生产达玛二烯二醇-II的毕赤酵母代谢工程。
J Biotechnol. 2015 Dec 20;216:47-55. doi: 10.1016/j.jbiotec.2015.10.005. Epub 2015 Oct 20.
8
Trm1p, a Zn(II)₂Cys₆-type transcription factor, is essential for the transcriptional activation of genes of methanol utilization pathway, in Pichia pastoris.Trm1p是一种Zn(II)₂Cys₆型转录因子，对巴斯德毕赤酵母中甲醇利用途径基因的转录激活至关重要。
Biochem Biophys Res Commun. 2014 Aug 15;451(1):158-64. doi: 10.1016/j.bbrc.2014.07.094. Epub 2014 Aug 1.
9
Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures.在葡萄糖-甲醇混合液中生长的重组蛋白分泌毕赤酵母的代谢通量谱分析。
Microb Cell Fact. 2012 May 8;11:57. doi: 10.1186/1475-2859-11-57.
10
Transcriptional Downregulation of Methanol Metabolism Key Genes During Yeast Death in Engineered Pichia pastoris.酵母毕赤酵母死亡过程中甲醇代谢关键基因的转录下调。
Biotechnol J. 2024 Oct;19(10):e202400328. doi: 10.1002/biot.202400328.

引用本文的文献

1
Synthetic polycistronic sequences in eukaryotes.真核生物中的合成多顺反子序列。
Synth Syst Biotechnol. 2021 Sep 15;6(4):254-261. doi: 10.1016/j.synbio.2021.09.003. eCollection 2021 Dec.

本文引用的文献

1
Exchanging the order of carotenogenic genes linked by porcine teschovirus-1 2A peptide enable to optimize carotenoid metabolic pathway in .交换由猪细小病毒1型2A肽连接的类胡萝卜素生成基因的顺序能够优化……中的类胡萝卜素代谢途径。 （原文句末不完整，缺少具体优化的对象）
RSC Adv. 2018 Oct 12;8(61):34967-34972. doi: 10.1039/c8ra06510a. eCollection 2018 Oct 10.
2
Metabolic engineering of Pichia pastoris.毕赤酵母的代谢工程。
Metab Eng. 2018 Nov;50:2-15. doi: 10.1016/j.ymben.2018.04.017. Epub 2018 Apr 25.
3
Cloning and high-level expression of β-xylosidase from Selenomonas ruminantium in Pichia pastoris by optimizing of pH, methanol concentration and temperature conditions.
通过优化pH、甲醇浓度和温度条件，在毕赤酵母中克隆并高效表达反刍月形单胞菌的β-木糖苷酶
Protein Expr Purif. 2016 Aug;124:55-61. doi: 10.1016/j.pep.2016.05.001. Epub 2016 May 3.
4
Δ10(E)-Sphingolipid Desaturase Involved in Fusaruside Mycosynthesis and Stress Adaptation in Fusarium graminearum.参与禾谷镰刀菌中镰刀菌素生物合成和胁迫适应的Δ10（E）-鞘脂去饱和酶
Sci Rep. 2015 May 21;5:10486. doi: 10.1038/srep10486.
5
Compact multi-enzyme pathways in P. pastoris.巴斯德毕赤酵母中的紧凑多酶途径。
Chem Commun (Camb). 2015 Jan 31;51(9):1643-6. doi: 10.1039/c4cc08502g.
6
PD-1/SHP-2 inhibits Tc1/Th1 phenotypic responses and the activation of T cells in the tumor microenvironment.程序性死亡受体1/含Src同源2结构域蛋白酪氨酸磷酸酶2抑制肿瘤微环境中细胞毒性T细胞1/辅助性T细胞1表型反应及T细胞活化。
Cancer Res. 2015 Feb 1;75(3):508-518. doi: 10.1158/0008-5472.CAN-14-1215. Epub 2014 Dec 5.
7
Synthetic biology tools for bioprospecting of natural products in eukaryotes.用于真核生物天然产物生物勘探的合成生物学工具。
Chem Biol. 2014 Apr 24;21(4):502-508. doi: 10.1016/j.chembiol.2014.02.010. Epub 2014 Mar 13.
8
Polycistronic expression of a β-carotene biosynthetic pathway in Saccharomyces cerevisiae coupled to β-ionone production.在酿酒酵母中β-胡萝卜素生物合成途径的多顺反子表达与β-紫罗兰酮的产生相关联。
J Biotechnol. 2014 Dec 20;192 Pt B:383-92. doi: 10.1016/j.jbiotec.2013.12.016. Epub 2014 Jan 28.
9
Simultaneous expression of multiple proteins under a single promoter in Caenorhabditis elegans via a versatile 2A-based toolkit.通过基于 2A 的多功能工具包在秀丽隐杆线虫中单启动子下同时表达多种蛋白质。
Genetics. 2014 Mar;196(3):605-13. doi: 10.1534/genetics.113.160846. Epub 2013 Dec 20.
10
Optimisation of the foot-and-mouth disease virus 2A co-expression system for biomedical applications.优化口蹄疫病毒 2A 共表达系统用于生物医学应用。
BMC Biotechnol. 2013 Aug 22;13:67. doi: 10.1186/1472-6750-13-67.