Wang Xuekun, Marchisio Mario Andrea
School of Pharmaceutical Science and Technology, Tianjin University, 92 Weijin Road, 300072, Tianjin, China.
Synth Syst Biotechnol. 2021 Sep 15;6(4):254-261. doi: 10.1016/j.synbio.2021.09.003. eCollection 2021 Dec.
The need for co-ordinate, high-level, and stable expression of multiple genes is essential for the engineering of biosynthetic circuits and metabolic pathways. This work outlines the functionality and design of IRES- and 2 A-peptide-based constructs by comparing different strategies for co-expression in polycistronic vectors. In particular, 2 A sequences are small peptides, mostly derived from viral polyproteins, that mediate a ribosome-skipping event such that several, different, separate proteins can be generated from a single open reading frame. When applied to metabolic engineering and synthetic gene circuits, 2 A peptides permit to achieve co-regulated and reliable expression of various genes in eukaryotic cells.
多个基因的协调、高水平和稳定表达对于生物合成电路和代谢途径的工程构建至关重要。这项工作通过比较多顺反子载体中不同的共表达策略，概述了基于内部核糖体进入位点（IRES）和2A肽的构建体的功能和设计。特别是，2A序列是小肽，大多源自病毒多聚蛋白，它们介导核糖体跳跃事件，从而可以从单个开放阅读框产生几种不同的、独立的蛋白质。当应用于代谢工程和合成基因电路时，2A肽能够在真核细胞中实现各种基因的共调控和可靠表达。
