Ocean College, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310014, PR China.
Ocean College, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310014, PR China.
J Biosci Bioeng. 2019 Dec;128(6):716-722. doi: 10.1016/j.jbiosc.2019.05.010. Epub 2019 Jun 14.
Aqueous enzymatic extraction of Camellia sinensis oil was studied. The results suggested that saponin removal pretreatment assisted by ultrasound was effective in decreasing emulsification and in enhancing the free oil recovery. After 70% isopropanol extraction for 30 min under ultrasound, the residue of C. sinensis seeds was further hydrolyzed with free cellulase and Alcalase for 5 h, and calcium ions were concurrently added during enzymatic hydrolysis (n: n = 1:2), and free oil recovery up to 94.14% was obtained. Separate immobilization and co-immobilization of Alcalase and cellulase were performed by alginate entrapment combined with glutaraldehyde crosslinking. Specific activity and recovery of activity for Alcalase and cellulase were acceptable. After immobilization, Alcalase and cellulase exhibited higher activity at a wider pH and temperature range. Reuse experiments of immobilized enzymes were conducted. The deactivation kinetics immobilized enzymes were simulated and half-life of immobilized enzyme was estimated. The results indicated that a magnetic supporter facilitated the recovery of immobilized enzymes from tea seed slurry, and that immobilized Alcalase and cellulase had good reusability.
对茶叶籽油的水酶法提取进行了研究。结果表明，超声波辅助的皂素去除预处理可有效降低乳化程度，提高游离油回收率。在超声波作用下用 70%异丙醇提取 30min 后，用游离纤维素酶和碱性蛋白酶对茶籽残渣进一步水解 5h，并在酶解过程中同时添加钙离子（n:n=1:2），可获得高达 94.14%的游离油回收率。采用海藻酸钠包埋结合戊二醛交联法对碱性蛋白酶和纤维素酶进行了分别固定化和共固定化。碱性蛋白酶和纤维素酶的比活和酶活回收率均在可接受的范围内。固定化后，Alcalase 和纤维素酶在更宽的 pH 和温度范围内表现出更高的活性。对固定化酶进行了重复使用实验。对固定化酶的失活动力学进行了模拟，并估算了固定化酶的半衰期。结果表明，磁性载体有利于从茶籽浆液中回收固定化酶，且固定化 Alcalase 和纤维素酶具有良好的可重复使用性。
