Camel Reproduction Centre, P.O. Box 79914, Dubai, United Arab Emirates.
Anim Reprod Sci. 2019 Aug;207:138-145. doi: 10.1016/j.anireprosci.2019.06.001. Epub 2019 Jun 4.
The use of camels for racing, milking and as show animals is growing in popularity, thus there is increased enthusiasm to breed more of the genetically superior animals. This review highlights recent developments in assisted reproductive techniques in camels, such as embryo transfer and artificial insemination, to ensure more rapid genetic progress. This paper discusses the difficulties involved in handling the semen due to its high viscosity and ways to reduce it. It also examines methods for short term liquid storage of fresh semen with and without the use of antioxidants to reduce oxidative stress. The widespread use of AI in camels is hindered by the lack of a reliable method for deep freezing and long term storage but various freezing protocols, cryoprotectants and freezing and thawing methods are discussed as well as different insemination techniques. Embryo transfer requires the donor to be superovulated and the recipients synchronized. This review discusses different protocols used for superovulation of donor animals and the problems involved. It also examines various methods to synchronize recipients, or how to make best use of non-synchronized or non ovulated recipients. Cryopreservation of embryos would greatly improve the wider use of ET and spread of genetics worldwide so methods for slow cooling and new methods of vitrification with promising results are discussed.
骆驼在赛马、产奶和表演动物方面的用途越来越受欢迎,因此人们对培育更多具有优良遗传特性的骆驼的热情日益高涨。本综述重点介绍了骆驼辅助生殖技术的最新进展,如胚胎移植和人工授精,以确保更快的遗传进展。本文讨论了由于精液高粘度而导致的处理困难,以及减少其粘度的方法。还研究了在有或没有抗氧化剂的情况下,短期储存新鲜精液的方法,以减少氧化应激。由于缺乏可靠的深冷冻和长期储存方法,AI 在骆驼中的广泛应用受到阻碍,但也讨论了各种冷冻方案、冷冻保护剂和冷冻和解冻方法,以及不同的授精技术。胚胎移植需要对供体进行超数排卵,并使受体同步。本文讨论了用于供体动物超数排卵的不同方案及其涉及的问题。还研究了各种使受体同步的方法,或如何充分利用非同步或未排卵的受体。胚胎的冷冻保存将极大地促进 ET 的更广泛应用和遗传资源的全球传播,因此讨论了缓慢冷却的方法和具有前景的玻璃化新方法。