Department of Embryology, Camel Advanced Reproductive Technologies Centre, Government of Dubai, Dubai, United Arab Emirates.
Methods Mol Biol. 2023;2647:283-303. doi: 10.1007/978-1-0716-3064-8_16.
Camels play very important economic and sociocultural roles for communities residing in arid and semi-arid countries. The positive impacts of cloning on genetic gain in camel species are indisputable, considering the unique ability of cloning to produce a large number of offspring of a predefined sex and genotype using somatic cells obtained from elite animals, live or dead, and within any age category. However, the current low efficiency of camel cloning seriously limits its commercial applicability. We have systematically optimized technical and biological factors for dromedary camel cloning. In this chapter, we present the details of our current standard operating procedure for dromedary camel cloning, namely, "modified handmade cloning (mHMC)."
骆驼在居住在干旱和半干旱国家的社区中发挥着非常重要的经济和社会文化作用。考虑到克隆技术独特的能力，可以使用来自精英动物的体细胞，无论是活体还是死体，并且在任何年龄段，产生大量预定性别和基因型的后代，克隆对骆驼物种的遗传增益的积极影响是不可争议的。然而，目前骆驼克隆的低效率严重限制了其商业适用性。我们已经系统地优化了骆驼克隆的技术和生物学因素。在本章中，我们介绍了我们当前的骆驼克隆标准操作程序的详细信息，即“改良手工克隆（mHMC）”。
