Kisielow P, Szkudlarek J, Wysocka M
Arch Immunol Ther Exp (Warsz). 1978;26(1-6):99-103.
Preparation of antisera to alloantigens Thy-1, TL, Lyt-1 and Lyt-2 is described and the importance of adequate specificity controls indicated. The specificity and activity of antisera were characterized by trypan blue exclusion cytotoxicity assay, indirect membrane immunofluorescence and absorption using cell populations from different tissues of Thy-1, TL and Lyt congenic strains of mice. In the course of these studies new serologically detectable Lyt-1 linked alloantigen was discovered which shows wide tissue distribution.
本文描述了针对同种异体抗原Thy-1、TL、Lyt-1和Lyt-2抗血清的制备方法，并指出了适当特异性对照的重要性。通过台盼蓝排斥细胞毒性试验、间接膜免疫荧光以及使用来自Thy-1、TL和Lyt同源系小鼠不同组织的细胞群体进行吸收试验，对抗血清的特异性和活性进行了表征。在这些研究过程中，发现了一种新的血清学可检测的与Lyt-1相关的同种异体抗原，其显示出广泛的组织分布。
