Pappa Eleni, Papadopoulos Savvas, Korou Laskarina-Maria, Perrea Despina N, Pneumaticos Spiridon, Nikolaou Vasileios S
"KAT" General Hospital of Athens, Nikis 2, 14561, Kifisia, Greece.
Department of Pathology, "Hygeia" General Hospital of Athens, Athens, Greece.
J Exp Orthop. 2019 Jun 17;6(1):25. doi: 10.1186/s40634-019-0194-4.
BACKGROUND: The purpose of this study is to investigate the possible attenuating role of the intra-articular administration of Fetuin-A in post-traumatic secondary osteoarthritis in rats, and also its effect on the systematic levels of interleukins (ILs)-2,4,7, bone morphogenetic proteins (BMPs) 2, 4, 7, C-Reactive Protein (CRP) and Fetuin-A.
Thirty male Sprague Dawley rats were separated in two groups where post-traumatic osteoarthritis was induced surgically by Anterior Cruciate Ligament Transection and the transection of the Medial Collateral Ligament of the right knee. In the Control Group, only the surgical intervention took place. In Fetuin Group, along with the induction of osteoarthritis, a single dose of bovine fetuin was administrated intra-articularly, intra-operatively. Both groups were examined for 8 weeks. The levels of interleukins, bone morphogenetic proteins, Fetuin-A and C-Reactive Protein were evaluated by ELISA of peripheral blood in three time periods: preoperatively, 5 and 8 weeks post-operatively. Osteoarthritic lesions of the knee were classified according to the Osteoarthritis Research Society International Grading System and the Modified Mankin Score, by histologic examination.
IL-2 levels were significantly decreased in the Fetuin Group. No statistical difference was signed on the levels of IL-7, BMP-2,4,7 and Fetuin-A between the two groups. CRP levels were significantly increased in the Fetuin Group in 5 weeks of the experiment. Fetuin Group signed better scores according to the OARSI classification system and Modified Mankin Score, without any statistical significance.
Intra-articular administration of Fetuin-A restrictively affected the progression of post-traumatic arthritis in rats, as only the levels of IL-2 were decreased as well as limited osteoarthritic lesions were observed on the Fetuin Group.
背景：本研究旨在探讨关节内注射胎球蛋白-A对大鼠创伤后继发性骨关节炎可能的减轻作用，以及其对白细胞介素（ILs）-2、4、7、骨形态发生蛋白（BMPs）2、4、7、C反应蛋白（CRP）和胎球蛋白-A系统水平的影响。
30只雄性Sprague Dawley大鼠被分为两组，通过切断右膝前交叉韧带和内侧副韧带手术诱导创伤后骨关节炎。对照组仅进行手术干预。在胎球蛋白组中，除诱导骨关节炎外，术中关节内注射单剂量牛胎球蛋白。两组均观察8周。在术前、术后5周和8周三个时间段，通过外周血酶联免疫吸附测定法评估白细胞介素、骨形态发生蛋白、胎球蛋白-A和C反应蛋白的水平。通过组织学检查，根据国际骨关节炎研究学会分级系统和改良曼金评分对膝关节的骨关节炎病变进行分类。
胎球蛋白组IL-2水平显著降低。两组间IL-7、BMP-2、4、7和胎球蛋白-A水平无统计学差异。在实验的第5周，胎球蛋白组CRP水平显著升高。根据骨关节炎研究学会国际分类系统和改良曼金评分，胎球蛋白组评分较好，但无统计学意义。
关节内注射胎球蛋白-A对大鼠创伤后关节炎的进展有一定限制作用，因为在胎球蛋白组中仅IL-2水平降低，且观察到有限的骨关节炎病变。
