Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China.
Environ Sci Pollut Res Int. 2019 Aug;26(23):23832-23841. doi: 10.1007/s11356-019-05660-7. Epub 2019 Jun 18.
The coastal environments worldwide are subjected to increasing TBBPA contamination, but current knowledge on aerobic biodegradability of this compound by marine microbes is lacking. The aerobic removal of TBBPA using marine consortia under eight different cometabolic conditions was investigated here. Results showed that the composition and diversity of the TBBPA-degrading consortia had diverged after 120-day incubation. Pseudoalteromonas, Alteromonas, Glaciecola, Thalassomonas, and Limnobacter were the dominant genera in enrichment cultures. Furthermore, a combination of beef extract- and peptone-enriched marine consortia exhibited higher TBBPA removal efficiency (approximately 60%) than the other substrate amendments. Additionally, Alteromonas macleodii strain GCW was isolated from a culture of TBBPA-degrading consortium. This strain exhibited about 90% of degradation efficiency toward TBBPA (10 mg L) after 10 days of incubation under aerobic cometabolic conditions. The intermediates in the degradation of TBBPA by A. macleodii strain GCW were analyzed and the degradation pathways were proposed, involving β-scission, debromination, and nitration routes.
全球沿海环境受到越来越多的四溴双酚 A（TBBPA）污染，但目前对于海洋微生物对该化合物好氧生物降解性的了解还很缺乏。本研究考察了在 8 种不同共代谢条件下利用海洋生物群落进行 TBBPA 好氧去除的情况。结果表明，经过 120 天的培养，TBBPA 降解生物群落的组成和多样性已经出现了分化。在富集培养物中，假交替单胞菌属、交替单胞菌属、冰海菌属、塔氏菌属和 Limnobacter 是优势属。此外，牛肉膏和蛋白胨强化的海洋生物群落组合比其他基质添加物表现出更高的 TBBPA 去除效率（约 60%）。此外，从 TBBPA 降解生物群落中分离到一株嗜冷杆菌（Alteromonas macleodii）GCW 菌株。该菌株在好氧共代谢条件下培养 10 天后，对 10mg/L 的 TBBPA 的降解效率约为 90%。分析了 A. macleodii 菌株 GCW 降解 TBBPA 的中间产物，并提出了降解途径，包括β断裂、脱溴和硝化途径。
