Environment Research Institute, Shandong University, Jinan 250100, China.
Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science & Engineering, Shandong University, Jinan 250100, China.
Ecotoxicol Environ Saf. 2018 Nov 30;164:597-603. doi: 10.1016/j.ecoenv.2018.08.053. Epub 2018 Aug 25.
Tetrabromobisphenol A (TBBPA) is the most widely used brominated flame retardant. Most studies regarding TBBPA have concentrated on its occurrence, distribution, toxicity and degradation in the environment. However, little is known about its ecological effects on soil microbial communities. In this study, we investigated the effect of TBBPA on soil microbial community. Overall, the data suggested that the growth and composition of soil microorganisms were correlated to the TBBPA concentration and exposure time. Phospholipid-derived fatty acid analysis (PLFAs) showed that significant microbial growth inhibitions were 46.1% and 46.9% in 40 mg/kg TBBPA-treated soils after 45-day incubation under aerobic and anaerobic conditions, respectively. Results of PLFAs and llumina sequencing indicated that TBBPA mainly inhibited Gram-positive bacteria, but not Gram-negative bacteria. The selective antibacterial activity of TBBPA toward Gram-positive bacteria was further confirmed in pure bacteria cultures. These data suggested that, in addition to their effect on microbial growth and composition, TBBPA may affect the microbial ecology. Additional research should be carried out to identify the ecological risk of TBBPA in soil.
四溴双酚 A（TBBPA）是目前使用最广泛的溴系阻燃剂。大多数关于 TBBPA 的研究都集中在其在环境中的存在、分布、毒性和降解方面。然而，对于其对土壤微生物群落的生态影响知之甚少。在这项研究中，我们调查了 TBBPA 对土壤微生物群落的影响。总的来说，数据表明土壤微生物的生长和组成与 TBBPA 浓度和暴露时间有关。磷脂衍生脂肪酸分析（PLFA）显示，在有氧和厌氧条件下分别在 40mg/kg TBBPA 处理的土壤中孵育 45 天后，微生物生长抑制率分别为 46.1%和 46.9%。PLFA 和 Illumina 测序的结果表明，TBBPA 主要抑制革兰氏阳性菌，但不抑制革兰氏阴性菌。在纯细菌培养中进一步证实了 TBBPA 对革兰氏阳性菌的选择性抑菌活性。这些数据表明，除了对微生物生长和组成的影响外，TBBPA 还可能影响微生物生态学。应开展更多研究，以确定土壤中 TBBPA 的生态风险。
