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[培养条件对生物体组织活力定量评估的影响]

[Effect of conditions of cultivation on quantitative assessment of the viability of an organism's tissues].

作者信息

Pashchenko V P

出版信息

Biull Eksp Biol Med. 1978 Nov;86(11):629-31.

PMID:31213
Abstract

Viability of mouse kidney tissue explants as estimated quantitatively according to their growth in plasmafree culture depends on some factors connected with cultivation conditions. Methods of serum preparation, pH of the medium, as well as peculiarities of tissue explants distribution in cultivation, their size and fastness of attachment to the substrate proved to influence the quantitative estimation of the tissue explants viability.

摘要

根据小鼠肾组织外植体在无血浆培养中的生长情况进行定量估计,其活力取决于一些与培养条件相关的因素。血清制备方法、培养基的pH值,以及组织外植体在培养中的分布特点、其大小和对底物的附着牢固性,都被证明会影响组织外植体活力的定量估计。

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