Marín-Padilla Miguel, Howard Louisa
Department of Pathology, The Geisel School of Medicine at Dartmouth, Hanover, NH, United States.
Front Neuroanat. 2019 Jun 3;13:49. doi: 10.3389/fnana.2019.00049. eCollection 2019.
In this article we explore filopodia of endothelial cells (ECs) in the developing central nervous system (CNS) using the Golgi method and transmission electron microscopy. Filopodia of ECs play a crucial role in the anastomosis of growing capillaries of the CNS. The leading ECs filopodia from approaching capillaries interconnect forming complex conglomerates that precede the anastomotic event. The contacting filopodia form narrow spaces between them filled with proteinaceous basal lamina material. The original narrow spaces coalesce into larger ones leading to the formation of a single one that will interconnect (anastomose) the two approaching capillaries. The four leading ECs (two for each approaching capillary) become the wall of the newly formed post-anastomotic CNS capillaries. These new CNS capillaries are very small with narrow and irregular lumina that might permit the passage of fluid but not yet of blood cells. Eventually, their lumen enlarges and permits the passage of blood cells.
在本文中，我们使用高尔基方法和透射电子显微镜研究发育中的中枢神经系统（CNS）中内皮细胞（EC）的丝状伪足。EC的丝状伪足在CNS生长中的毛细血管吻合中起关键作用。来自接近毛细血管的领先EC丝状伪足相互连接，形成在吻合事件之前的复杂聚集体。接触的丝状伪足在它们之间形成充满蛋白质性基底膜物质的狭窄空间。原始的狭窄空间合并成更大的空间，导致形成一个将连接（吻合）两个接近毛细血管的单一空间。四个领先的EC（每个接近的毛细血管两个）成为新形成的吻合后CNS毛细血管的壁。这些新的CNS毛细血管非常小，管腔狭窄且不规则，可能允许液体通过，但尚未允许血细胞通过。最终，它们的管腔扩大并允许血细胞通过。
