Rice Research Institute, Key Laboratory of Application and Safety Control of Genetically Modified Crops, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Transgenic Res. 2019 Aug;28(3-4):381-390. doi: 10.1007/s11248-019-00158-x. Epub 2019 Jun 18.
No usable resources with high-level resistance to sheath blight (SB) have yet been found in rice germplasm resources worldwide. Therefore, creating and breeding new disease-resistant rice resources with sheath blight resistance (SBR) are imperative. In this study, we inoculated rice plants with hyphae of the highly pathogenic strain RH-9 of rice SB fungus Rhizoctonia solani to obtain eight stable transgenic rice lines harbouring the chitinase gene (McCHIT1) of bitter melon with good SBR in the T generation. The mean disease index for SB of wild-type plants was 92% and 37-44% in transgenic lines. From 24 h before until 120 h after inoculation with R. solani, chitinase activity in stable transgenic plants with increased SBR was 2.0-5.5 and 1.8-2.7 times that of wild-type plants and plants of a disease-susceptible stable transgenic line, respectively. The correlation between SBR and chitinase activity in McCHIT1-transgenic rice line plants was significant. This work stresses how McCHIT1 from bitter melon can be used to protect rice plants from SB infection.
目前在全球范围内，尚未在水稻种质资源中发现对纹枯病（SB）具有高水平抗性的可用资源。因此，创造和培育具有纹枯病抗性（SBR）的新抗病性水稻资源势在必行。本研究通过接种高致病性 RH-9 菌株的水稻纹枯病菌丝，获得了 8 个稳定表达苦瓜几丁质酶基因（McCHIT1）的转 McCHIT1 基因水稻株系，在 T 代具有良好的 SBR。野生型植株的纹枯病平均发病率为 92%，转 McCHIT1 基因的植株为 37-44%。从接种 Rhizoctonia solani 前 24 小时到接种后 120 小时，SBR 增强的稳定转 McCHIT1 植物的几丁质酶活性分别是野生型和易感病稳定转 McCHIT1 植物的 2.0-5.5 倍和 1.8-2.7 倍。McCHIT1 转基因水稻植株的 SBR 与几丁质酶活性之间存在显著相关性。这项工作强调了苦瓜中的 McCHIT1 如何用于保护水稻植株免受 SB 感染。
