Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, College of Animal Science and Technology, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, China.
Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, College of Animal Science and Technology, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, China.
Fish Shellfish Immunol. 2019 Sep;92:348-355. doi: 10.1016/j.fsi.2019.06.034. Epub 2019 Jun 16.
This is the first study to use a next-generation high-throughput DNA sequencing technique, the Illumina Hiseq2000 method, to analyze the transcriptome from hemocytes of Marsupenaeus japonicus. A total of 80,929,652 Illumina reads, including 79,525,942 high quality reads were obtained in this study. From these, 40,231 unigenes with a mean length of 1557 bp were assembled using Trinity de novo software and 28,746 cDNA were matched in the NCBI database. Then we compared the transcriptome changes after white spot syndrome virus (WSSV) or Vibrio alginolyticus infection. A total of 19,872 putative proteins were classified functionally into 25 molecular families in the cluster of orthologous groups. KEGG pathway analysis identified that the metabolic pathway possessed more unigenes (1358 unigenes), followed by biosynthesis of secondary metabolites, Huntington's disease and RNA transport. Important immune functions like apoptosis, phagocytosis, and lysosomes were in response to WSSV and V. alginolyticus early infection. Only 26 transcripts were significantly up-regulated or down-regulated after WSSV infection showed compared with V. alginolyticus. Crustin-like protein, endonuclease-reverse transcriptase, putative nuclease HARBI1-like, diphthamide biosynthesis protein 7 and hormone receptor 3 were involved in the immune response to WSSV or V. alginolyticus infection. These transcriptome datasets accelerate our understanding of the innate immune mechanisms in M. japonicus and other crustaceans.
这是首次使用高通量 DNA 测序技术（Illumina Hiseq2000 方法）来分析日本沼虾血细胞转录组的研究。在本研究中，共获得了 80929652 个 Illumina 读取序列，其中包括 79525942 个高质量读取序列。利用 Trinity de novo 软件组装这些序列，共得到 40231 条平均长度为 1557bp 的 unigenes，其中 28746 条 cDNA 在 NCBI 数据库中得到匹配。然后，我们比较了白斑综合征病毒（WSSV）或溶藻弧菌感染后的转录组变化。共鉴定出 19872 个假定蛋白，这些蛋白被分为 25 个分子家族，聚类于同源基因。KEGG 通路分析发现代谢途径具有更多的 unigenes（1358 个），其次是次生代谢物的生物合成、亨廷顿病和 RNA 转运。凋亡、吞噬和溶酶体等重要的免疫功能在 WSSV 和 V. alginolyticus 早期感染中都有反应。与 V. alginolyticus 感染相比，WSSV 感染后仅有 26 个转录本显著上调或下调。几丁质结合蛋白、内切酶-逆转录酶、假定核酸内切酶 HARBI1 样蛋白、二氢叶酸生物合成蛋白 7 和激素受体 3 参与了对 WSSV 或 V. alginolyticus 感染的免疫反应。这些转录组数据集加速了我们对日本沼虾及其他甲壳动物先天免疫机制的理解。
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