Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, College of Animal Science and Technology, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, China.
Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, College of Animal Science and Technology, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, China.
Fish Shellfish Immunol. 2019 Apr;87:297-306. doi: 10.1016/j.fsi.2019.01.028. Epub 2019 Jan 25.
Relish is a transcription factor and forms an important part of the immune deficiency signaling pathway. In the current study, a Relish homolog was cloned from the hemolymph of Scylla paramamosain using RT-PCR and RACE. The full length cDNA of Relish consists of 4263 base pairs (bp), including a 3552 bp open reading frame encoding a 1184 amino acid protein. The data showed that Relish was highly expressed in the gonad and digestive organs of S. paramamosain. Furthermore, the expression of Relish was up-regulated by infection with white spot syndrome virus (WSSV) or Vibrio alginolyticus. When Relish was knocked down, immune genes such as Janus Kinase, signal transducer and activator of transcription, crustin antimicrobial peptide, prophenoloxidase, C-type-lectin and myosin-II-essential-light-chain-like-protein were significantly down-regulated (P < 0.01), and Toll-like receptor was significantly up-regulated (P < 0.01) in hemocytes. The mortality of WSSV-infected or V. alginolyticus-infected crabs was enhanced following Relish knockdown. Thus, Relish is very important in the progression of WSSV and V. alginolyticus infection. It was found that Relish knockdown caused the highest level of apoptosis in the disease-free group, and higher levels of apoptosis in the WSSV group and V. alginolyticus group compared with that in the control group. Knockdown of Relish influenced the activity of phenoloxidase (PO) and superoxide dismutase (SOD), and total hemocyte count (THC) following WSSV or V. alginolyticus infection, indicating that Relish plays a regulatory role in the immune response to WSSV or V. alginolyticus infection in crabs. Thus, we conclude that Relish may anticipate host defense mechanisms against pathogen infection by affecting apoptosis, THC, PO activity and SOD activity.
relish 是一种转录因子，是免疫缺陷信号通路的重要组成部分。在本研究中，我们通过 RT-PCR 和 RACE 从锯缘青蟹的血淋巴中克隆了 relish 同源物。Relish 的全长 cDNA 由 4263 个碱基对（bp）组成，包括一个编码 1184 个氨基酸的蛋白质的 3552 bp 开放阅读框。数据显示，Relish 在锯缘青蟹的性腺和消化器官中高度表达。此外，Relish 的表达在感染白斑综合征病毒（WSSV）或溶藻弧菌后上调。当 Relish 被敲低时，Janus 激酶、信号转导和转录激活因子、抗菌肽 crustin、酚氧化酶原、C 型凝集素和肌球蛋白-II-必需轻链样蛋白等免疫基因显著下调（P < 0.01），而 Toll 样受体显著上调（P < 0.01）在血细胞中。Relish 敲低后，WSSV 感染或溶藻弧菌感染蟹的死亡率增加。因此，Relish 在 WSSV 和 V. alginolyticus 感染的进展中非常重要。研究发现，Relish 敲低在无病组引起的细胞凋亡水平最高，WSSV 组和溶藻弧菌组的细胞凋亡水平均高于对照组。Relish 敲低影响酚氧化酶（PO）和超氧化物歧化酶（SOD）的活性以及总血细胞计数（THC）在 WSSV 或 V. alginolyticus 感染后，表明 Relish 在蟹对 WSSV 或 V. alginolyticus 感染的免疫反应中起调节作用。因此，我们得出结论，Relish 可能通过影响细胞凋亡、THC、PO 活性和 SOD 活性来预测宿主防御机制对病原体感染的反应。
