Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
Fish Shellfish Immunol. 2019 Apr;87:755-764. doi: 10.1016/j.fsi.2019.02.030. Epub 2019 Feb 18.
Vibrio parahaemolyticus and white spot syndrome virus (WSSV) are pathogens that cause epidemics in kuruma shrimp (Marsupenaeus japonicus) during aquaculture, resulting in severe economic losses to local farmers. To characterise the mechanisms of the molecular responses to V. parahaemolyticus and WSSV infection in M. japonicus, the transcriptome of hepatopancreas was sequenced using next-generation sequencing after infection. A total of 29,180 unigenes were assembled, with an average length of 1,151 bp (N50 = 1,951 bp). After BLASTX searching against the Nr database (E-value cut-off = 10), 15,176 assembled unigenes remained, with 3,039 and 1,803 differentially expressed transcripts identified in the V. parahaemolyticus- and WSSV-infected groups, respectively. Of these, 1466 transcripts were up-regulated and 1573 were down-regulated in V. parahaemolyticus-infected shrimps, and 970 transcripts were up-regulated and 833 were down-regulated in the WSSV-infected shrimps. Additionally, 761 transcripts were differentially expressed in both V. parahaemolyticus- and WSSV-infected shrimps. Several known immune-related genes including caspase 4, integrin, crustin, ubiquitin-conjugating enzyme E2, C-type lectin, and α-macroglobulin were among the differentially expressed transcripts. These results provide valuable information for characterising the immune mechanisms of the shrimp responses of to V. parahaemolyticus andWSSV infection.
副溶血弧菌和白斑综合征病毒（WSSV）是水产养殖中对日本对虾（Marsupenaeus japonicus）造成流行性病的病原体，给当地养殖户造成严重的经济损失。为了研究日本对虾对副溶血弧菌和 WSSV 感染的分子应答机制，在感染后使用下一代测序技术对其肝胰腺转录组进行了测序。共组装了 29180 个 unigenes，平均长度为 1151bp（N50=1951bp）。经过与 Nr 数据库（E 值截止值=10）的 BLASTX 搜索，剩余 15176 个组装的 unigenes，在副溶血弧菌和 WSSV 感染组中分别鉴定出 3039 个和 1803 个差异表达的转录本。其中，副溶血弧菌感染的虾中 1466 个转录本上调，1573 个转录本下调；WSSV 感染的虾中 970 个转录本上调，833 个转录本下调。此外，761 个转录本在副溶血弧菌和 WSSV 感染的虾中均有差异表达。几种已知的免疫相关基因，包括半胱天冬酶 4、整合素、甲壳质、泛素结合酶 E2、C 型凝集素和α-巨球蛋白，都属于差异表达的转录本。这些结果为研究日本对虾对副溶血弧菌和 WSSV 感染的免疫机制提供了有价值的信息。
