Zhang Yu, Yang Jing, Wang Jiayi, Chen Ling, Huang Huiming, Xiong Yuanzhen, Xie Baogang
Department of Pharmaceutics, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Bioanalysis. 2019 May;11(10):1003-1013. doi: 10.4155/bio-2018-0319.
In order to differential diagnosis of chronic hepatitis B (HBV-I) and hepatocellular carcinoma (HCC), a UPLC-MS/MS method for measuring purine metabolites was developed. serum samples from 26 HBV-I and 35 HCC patients were collected. Ten purine metabolites were simultaneously quantified by UPLC-MS/MS with tubercidin and uric acid-1,3-N as internal standards. The method was validated to meet the requirements of clinical sample analysis. A logistic equation was established for differential diagnosis of HBV-I and HCC by combination of xanthosine and guanine with the area under the receiver operating characteristic curve of 0.885. Guanine and xanthosine are intermediates in the metabolism of purine, which play an important role in gene synthesis, and metabolism regulation. The alteration of serum purine metabolite may contribute to differential diagnosis of HBV-I and HCC.
为鉴别诊断慢性乙型肝炎（HBV-I）和肝细胞癌（HCC），建立了一种测定嘌呤代谢物的超高效液相色谱-串联质谱（UPLC-MS/MS）方法。收集了26例HBV-I患者和35例HCC患者的血清样本。以杀结核菌素和尿酸-1,3-N为内标，通过UPLC-MS/MS同时定量10种嘌呤代谢物。该方法经验证符合临床样本分析要求。通过将黄苷和鸟嘌呤相结合建立了用于HBV-I和HCC鉴别诊断的逻辑方程，受试者工作特征曲线下面积为0.885。鸟嘌呤和黄苷是嘌呤代谢的中间产物，在基因合成和代谢调节中起重要作用。血清嘌呤代谢物的改变可能有助于HBV-I和HCC的鉴别诊断。
