Department of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, (Westville Campus), Durban, 4000, South Africa; Nutrition and Toxicology Division, Federal Institute of Industrial Research, Lagos, Nigeria.
Department of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, (Westville Campus), Durban, 4000, South Africa.
J Ethnopharmacol. 2019 Oct 5;242:112033. doi: 10.1016/j.jep.2019.112033. Epub 2019 Jun 18.
Cola nitida is amongst the evergreen plants native to West Africa used in the treatment of various ailments including diabetes.
This study aims to investigate the antidiabetic effects of the hot water extract of C. nitida seeds in type 2 diabetic rats.
Type 2 diabetic rats were orally administered with low (150 mg/kg bw) and high (300 mg/kg bw) doses of the hot water extract for 6 wk and thereafter, blood glucose, insulin level, lipid profile, pancreatic β-cell function, perfusion and morphology, redox imbalance, glycolytic and cholinergic enzymes, as well as of caspase-3 and Nrf2 expressions were measured.
Treatment with the extract led to significant depletion of blood glucose, serum triglycerides, LDL-cholesterol, fructosamine, ALT, and uric acids, while elevating serum insulin and HDL-cholesterol levels. The infusion also significantly (p < 0.05) elevated GSH level, SOD, catalase, α-amylase, and ATPase activities, with concomitant depletion of myeloperoxidase enzyme activity, and NO and MDA levels in the serum and pancreas. Significantly (p < 0.05) improved pancreatic β-cell function and morphology were observed in rats treated with C. nitida, with restored pancreatic capillary networks. C. nitida inhibited the activities of glycogen phosphorylase, fructose 1,6 biphosphatase, glucose 6 phosphatase, and acetylcholinesterase while downregulated the Nrf2 expression. NMR analysis of the extract revealed the presence of caffeine and theobromine. The molecular docking studies indicated that identified compounds displayed strong molecular interactions with caspase-3 and Nrf2.
These results insinuate the antidiabetic activities of C. nitida hot water extract and may be attributed to the NMR-identified compounds.
非洲西部的常绿树可乐果被用于治疗各种疾病，包括糖尿病，是其本土植物之一。
本研究旨在探讨可乐果种子热水提取物对 2 型糖尿病大鼠的降血糖作用。
2 型糖尿病大鼠口服给予低（150mg/kg bw）和高（300mg/kg bw）剂量的热水提取物 6 周，然后测量血糖、胰岛素水平、血脂谱、胰岛β细胞功能、灌注和形态、氧化还原失衡、糖酵解和胆碱能酶以及 caspase-3 和 Nrf2 的表达。
提取物的治疗导致血糖、血清三酰甘油、LDL-胆固醇、果糖胺、ALT 和尿酸显著减少，而血清胰岛素和 HDL-胆固醇水平升高。该输注还显著（p<0.05）提高了 GSH 水平、SOD、过氧化氢酶、α-淀粉酶和 ATP 酶活性，同时降低了血清和胰腺中的髓过氧化物酶活性、NO 和 MDA 水平。用可乐果治疗的大鼠观察到胰岛β细胞功能和形态显著改善（p<0.05），恢复了胰腺毛细血管网络。可乐果抑制了糖原磷酸化酶、果糖 1,6 二磷酸酶、葡萄糖 6 磷酸酶和乙酰胆碱酯酶的活性，同时下调了 Nrf2 的表达。提取物的 NMR 分析表明存在咖啡因和可可碱。分子对接研究表明，鉴定出的化合物与 caspase-3 和 Nrf2 显示出强烈的分子相互作用。
这些结果暗示了可乐果热水提取物的抗糖尿病活性，可能归因于 NMR 鉴定的化合物。
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