Fishery College, Zhejiang Ocean University, 1st Haidanan Road, Zhoushan, 316022, P.R. China.
The First Institute of Oceanography, Ministry of Natural Resources, 6th Xianxialing Road, Qingdao, 266061, P.R. China.
Sci Data. 2019 Jun 20;6(1):97. doi: 10.1038/s41597-019-0100-z.
Here we report Illumina-based whole genome sequencing of three rockfish species of Sebastes in northwest Pacific. The whole genomic DNA was used to prepare 350-bp pair-end libraries and the high-throughput sequencing yielded 128.5, 137.5, and 124.8 million mapped reads corresponding to 38.54, 41.26, and 37.43 Gb sequence data for S. schlegelii, S. koreanus, and S. nudus, respectively. The k-mer analyses revealed genome sizes were 846.4, 832.5, and 813.1 Mb and the sequencing coverages were 45×, 49×, and 46× for three rockfish, respectively. Comparative genomic analyses identified 46,624 genome-wide single nucleotide polymorphisms (SNPs). Phylogenetic analysis revealed closer relationships of the three species, compared to other six rockfish species. Demographic analysis identified contrasting changes between S. schlegelii and other two species, suggesting drastically different response to climate changes. The reported genome data in this study are valuable for further studies on comparative genomics and evolutionary biology of rockfish species.
我们在此报告了西北太平洋三种石斑鱼属（Sebastes）鱼类的基于 Illumina 的全基因组测序。使用全基因组 DNA 制备了 350-bp 双端文库，高通量测序产生了 128.5、137.5 和 124.8 百万个映射reads，分别对应于 S. schlegelii、S. koreanus 和 S. nudus 的 38.54、41.26 和 37.43 Gb 序列数据。k-mer 分析表明基因组大小分别为 846.4、832.5 和 813.1 Mb，测序覆盖率分别为 45×、49×和 46×。比较基因组分析鉴定了 46624 个全基因组单核苷酸多态性（SNP）。系统发育分析表明，与其他六种石斑鱼相比，这三个物种之间的亲缘关系更为密切。种群动态分析鉴定了 S. schlegelii 与其他两个物种之间的变化存在差异，表明它们对气候变化的反应截然不同。本研究中报告的基因组数据对于进一步研究石斑鱼属物种的比较基因组学和进化生物学具有重要价值。
