MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Mol Ecol Resour. 2019 Sep;19(5):1309-1321. doi: 10.1111/1755-0998.13034. Epub 2019 Jul 9.
The black rockfish (Sebastes schlegelii) is a teleost in which eggs are fertilized internally and retained in the maternal reproductive system, where they undergo development until live birth (viviparity). In the present study, we report a chromosome-level black rockfish genome assembly. High-throughput transcriptome analysis (RNA-seq and ATAC-seq) coupled with in situ hybridization (ISH) and immunofluorescence reveal several candidate genes for maternal preparation, sperm storage and release, and hatching. We propose that zona pellucida (ZP) proteins retain sperm at the oocyte envelope, while genes in two distinct astacin metalloproteinase subfamilies serve to release sperm from the ZP and free the embryo from chorion at prehatching stage. We present a model of black rockfish reproduction, and propose that the rockfish ovarian wall has a similar function to the uterus of mammals. Together, these genomic data reveal unprecedented insights into the evolution of an unusual teleost life history strategy, and provide a sound foundation for studying viviparity in nonmammalian vertebrates and an invaluable resource for rockfish ecological and evolutionary research.
黑鮟鱇鱼（Sebastes schlegelii）是一种硬骨鱼，其卵子在体内受精并保留在母体生殖系统中，在那里发育直至活体分娩（胎生）。在本研究中，我们报告了一个染色体水平的黑鮟鱇鱼基因组组装。高通量转录组分析（RNA-seq 和 ATAC-seq）结合原位杂交（ISH）和免疫荧光揭示了几个候选基因，用于母体准备、精子储存和释放以及孵化。我们提出，透明带（ZP）蛋白将精子保留在卵母细胞的包膜上，而两个不同的星状丝氨酸蛋白酶亚家族的基因则用于从 ZP 释放精子，并在孵化前阶段将胚胎从绒毛膜中释放出来。我们提出了黑鮟鱇鱼生殖的模型，并提出了鱼类卵巢壁具有类似于哺乳动物子宫的功能。总之，这些基因组数据揭示了对一种不寻常的硬骨鱼生活史策略进化的前所未有的见解，并为研究非哺乳动物脊椎动物的胎生提供了坚实的基础，同时为鱼类生态和进化研究提供了宝贵的资源。
