Denisenko M F, Belovskaia L N, Soldatenkov V A, Smirnova T N, Filippovich I V
Radiobiologiia. 1987 Nov-Dec;27(6):737-42.
A decrease in the endogenous NAD content that occurs immediately after gamma-irradiation of thymus lymphocytes is attributed to activation of poly (ADP-ribosylation) and not to changes in the activity of NAD-glycohydrolase and/or to the release of NAD from cells. The addition of benzamide 60 min before irradiation prevents the postirradiation drop of the NAD level and produces a radioprotective effect. At the same time, benzamide inhibits nuclear superhelix DNA repair and causes an average of 40 per cent decrease in the activity of DNA ligases I and II. The authors discuss the idea that the content of intracellular NAD in thymocytes is a critical factor responsible for the vitality of these cells.
胸腺淋巴细胞经γ射线照射后，内源性烟酰胺腺嘌呤二核苷酸（NAD）含量立即下降，这归因于多聚（ADP - 核糖基化）的激活，而不是NAD - 糖苷水解酶活性的变化和/或NAD从细胞中的释放。照射前60分钟添加苯甲酰胺可防止照射后NAD水平下降，并产生辐射防护作用。同时，苯甲酰胺抑制核超螺旋DNA修复，并使DNA连接酶I和II的活性平均降低40%。作者讨论了胸腺细胞内NAD含量是这些细胞活力关键因素的观点。
