替普瑞酮通过抑制一氧化氮的产生来减轻神经性疼痛。
Tempol Attenuates Neuropathic Pain by Inhibiting Nitric Oxide Production.
机构信息
Department of Pain Medicine, Peking University Third Hospital, Beijing 100191, China.
Department of Anesthesiology, Gongli Hospital, The Second Military Medical University, Pudong New District, Shanghai 200135, China.
出版信息
Anal Cell Pathol (Amst). 2019 May 15;2019:8253850. doi: 10.1155/2019/8253850. eCollection 2019.
BACKGROUND
Neuropathic pain not only affects individual life quality but also increases economic burden for the society. Treatment to alleviate neuropathic pain is required.
METHODOLOGY
Fifty rats were randomly assigned into sham, spinal nerve ligation, and three treatment groups with different doses of Tempol (100, 200, and 300 mg/kg, respectively), with 10 rats in each group. A neuropathic pain model was created with spinal nerve L5 and L6 ligation. Mechanical allodynia and thermal hyperalgesia were tested preoperatively (day 0) and postoperatively (days 1, 3, 5, and 7). Spinal cord levels of nitric oxide, as well as activities of nitric oxide synthase and acetylcholinesterase, were tested in postoperative day 7.
RESULTS
Compared with rats in the spinal nerve ligation group, rats in Tempol treatment groups had decreased responses to mechanical pain and cold plate stimulations. A high dose of Tempol produced more attenuating effects. The level of nitric oxide and activity of nitric oxide synthase were also decreased with Tempol treatments, whereas no significant changes were observed in the activity of acetylcholinesterase.
CONCLUSIONS
Tempol attenuated an experimental rat model with neuropathic pain by inhibiting nitric oxide production.
背景
神经病理性疼痛不仅影响个体的生活质量，还会增加社会的经济负担。因此需要对其进行治疗。
方法
将 50 只大鼠随机分为假手术组、脊神经结扎组和三个治疗组，每组 10 只，分别给予不同剂量的 Tempol（100、200 和 300mg/kg）。用 L5 和 L6 脊神经结扎法建立神经病理性疼痛模型。术前（第 0 天）和术后（第 1、3、5 和 7 天）检测机械性痛觉过敏和热痛觉过敏。术后第 7 天检测脊髓一氧化氮水平以及一氧化氮合酶和乙酰胆碱酯酶的活性。
结果
与脊神经结扎组大鼠相比，Tempol 治疗组大鼠对机械性疼痛和冷板刺激的反应减轻，高剂量 Tempol 的抑制作用更强。Tempol 治疗还降低了一氧化氮水平和一氧化氮合酶活性，而乙酰胆碱酯酶的活性无明显变化。
结论
Tempol 通过抑制一氧化氮的产生来减轻神经病理性疼痛的实验性大鼠模型。
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