Eneström S
Department of Pathology I, Linköping University, Sweden.
Stain Technol. 1987 Sep;62(5):327-33. doi: 10.3109/10520298709108017.
Correlative morphology of kidney tissue can be accomplished by a combination of immunofluorescent staining and light microscopy using a pre-embedding technique. Further development of this method has been elaborated by freeze-drying and chopping of the tissue, which then is incubated in biotinylated immunoglobulins. Fluorochromes and colloidal gold can be coupled to the biotinylated immunoglobulins in a second step. This technique is an alternative to postembedding immunogold staining for immunoelectron microscopy, and permits a correlative immunohistochemical and detailed morphological study of kidney tissue.
肾脏组织的相关形态学研究可通过免疫荧光染色和使用包埋前技术的光学显微镜检查相结合来完成。通过对组织进行冷冻干燥和切片，进一步改进了该方法，然后将其置于生物素化免疫球蛋白中孵育。在第二步中，荧光染料和胶体金可与生物素化免疫球蛋白偶联。该技术是免疫电子显微镜包埋后免疫金染色的替代方法，可对肾脏组织进行相关的免疫组织化学和详细的形态学研究。
