Department of Chemical Engineering, Visvesvaraya National Institute of Technology, South Ambazari Road, Nagpur, MS, 440010, India.
Appl Biochem Biotechnol. 2019 Dec;189(4):1209-1222. doi: 10.1007/s12010-019-03069-z. Epub 2019 Jun 22.
The foremost aim of the present study was to enhance butanol production in an extractive fermentation study in presence of non-ionic surfactant using immobilized cells. Earlier studies had shown improved butanol production with non-ionic surfactant and immobilized cells independently. Therefore, in the present work, the combined effect of extractive fermentation and immobilized cells on butanol production was studied. Different matrices (brick, bamboo, cotton fiber, flannel cloth, and polyurethane foam) were tested for immobilization of Clostridium sporogenes. Immobilized biomass thus obtained was used in an extractive fermentation study with non-ionic surfactant L62. Biomass immobilized on polyurethane foam (PF) doubled the butanol production in presence of 6% (v/v) L62 with respect to control (free cells without surfactant). Further, the effect of different carbon sources was also studied to check the suitability of different industrial wastes containing different carbon sources. Glucose was found to be the best carbon source.
本研究的首要目的是在使用固定化细胞的萃取发酵研究中提高丁醇的产量。早期的研究表明，非离子表面活性剂和固定化细胞都可以提高丁醇的产量。因此，在本工作中，研究了萃取发酵和固定化细胞对丁醇生产的综合影响。不同的基质（砖、竹、棉纤维、绒布和聚氨酯泡沫）被用于固定凝结芽孢杆菌。所得固定化生物量用于非离子表面活性剂 L62 的萃取发酵研究。与对照（无表面活性剂的游离细胞）相比，在 6%（v/v）L62 存在下，固定在聚氨酯泡沫（PF）上的生物量使丁醇产量增加了一倍。此外，还研究了不同碳源的影响，以检查不同工业废物中不同碳源的适用性。发现葡萄糖是最佳的碳源。
