Singh Kajal, Gedam Preety S, Raut Atulkumar N, Dhamole Pradip B, Dhakephalkar P K, Ranade Dilip R
MACS-Agharkar Research Institute (ARI), G.G. Agharkar Road, Pune, Maharashtra, 411004, India.
Department of Chemical Engineering, Visvesvaraya National Institute of Technology, South Ambazari Road, Nagpur, Maharashtra, 440010, India.
3 Biotech. 2017 Jul;7(3):161. doi: 10.1007/s13205-017-0803-9. Epub 2017 Jun 29.
Extractive butanol fermentation with non-ionic surfactant, a recently explored area, has shown promising results with several advantages but is relatively less investigated. This work reports the extractive fermentation with selected non-ionic surfactants (L62 and L62D) to enhance butanol production using a high-butanol producing strain (Clostridium beijerinckii MCMB 581). Biocompatibility studies with both the surfactants showed growth. Higher concentrations of surfactant (>5%) affected the cell count. 15.3 g L of butanol and 21 g L of total solvents were obtained with 3% (v/v) L62 which was respectively, 43% (w/w) and 55% (w/w), higher than control. It was found that surfactant addition at 9th h doubled the productivity (from 0.13 to 0.31 g L h and 0.17 to 0.39 g L h, respectively for butanol and total solvent). Butanol productivity obtained was 2-3 times higher than similar studies on extractive fermentation with non-ionic surfactants. Interestingly, mixing did not improve butanol production.
采用非离子表面活性剂进行萃取丁醇发酵是一个最近才探索的领域，已显示出有前景的结果，具有几个优点，但相对较少受到研究。这项工作报道了使用选定的非离子表面活性剂（L62和L62D）进行萃取发酵，以利用高产丁醇菌株（拜氏梭菌MCMB 581）提高丁醇产量。对这两种表面活性剂的生物相容性研究显示有生长现象。较高浓度的表面活性剂（>5%）影响细胞计数。使用3%（v/v）的L62获得了15.3 g/L的丁醇和21 g/L的总溶剂，分别比对照高43%（w/w）和55%（w/w）。发现第9小时添加表面活性剂使生产率提高了一倍（丁醇和总溶剂的生产率分别从0.13 g/L·h和0.17 g/L·h提高到0.31 g/L·h和0.39 g/L·h）。所获得的丁醇生产率比关于非离子表面活性剂萃取发酵的类似研究高2至3倍。有趣的是，混合并没有提高丁醇产量。
