Faculty of Advanced Life Science, Hokkaido University, Sapporo, Japan.
Methods Mol Biol. 2020;2109:199-208. doi: 10.1007/7651_2019_245.
Applications of RNA sequencing have been wide-spreading in various subfields of life science. Construction of information and statistical analysis pipeline is indispensable to process raw RNA sequencing (RNA-seq) data generated by next-generation sequencers in order to extract biological implications. In this chapter, we introduce a common pipeline for RNA-seq data. A collection of notes on related advanced topics will be useful when conducting information and statistical analysis in practice.
RNA 测序的应用已经广泛扩展到生命科学的各个领域。为了提取生物学意义，必须构建信息和统计分析管道来处理下一代测序仪生成的原始 RNA 测序 (RNA-seq) 数据。在本章中，我们介绍了一种常见的 RNA-seq 数据管道。在进行信息和统计分析时，相关高级主题的注释集将非常有用。
