大鼠胚胎干细胞与基因操作

Embryonic Stem Cells and Gene Manipulation in Rat.

作者信息

Hirabayashi Masumi, Takizawa Akiko, Hochi Shinichi

机构信息

Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Aichi, Japan.

Genomic Sciences and Precision Medicine Center, Medical College of Wisconsin, Milwaukee, WI, USA.

出版信息

Methods Mol Biol. 2019;2018:115-130. doi: 10.1007/978-1-4939-9581-3_5.

DOI:10.1007/978-1-4939-9581-3_5

PMID:31228154

Abstract

Transgenic technology in rats is increasingly important for the design and implementation of biological and physiological studies in the fields of neuroscience, pharmacology, and toxicology. Pluripotent embryonic stem cells (ESCs) are a useful tool for generation of gene-modified rats. During the last decade, not only foreign DNA introduction but also endogenous DNA modification has been successfully achieved with rat ESCs. Detailed protocols for establishment of bona fide rat ESCs and their use for production of gene-modified rats are described in this chapter.

摘要

转基因技术在大鼠中对于神经科学、药理学和毒理学领域的生物学和生理学研究的设计与实施日益重要。多能胚胎干细胞（ESCs）是生成基因修饰大鼠的有用工具。在过去十年中，不仅外源DNA导入，而且大鼠ESCs的内源DNA修饰也已成功实现。本章描述了建立真正的大鼠ESCs及其用于生产基因修饰大鼠的详细方案。