微粉碎脂肪来源的脂肪间充质干细胞在杜氏肌营养不良症小鼠模型中的有益作用。
Beneficial role of adipose-derived mesenchymal stem cells from microfragmented fat in a murine model of duchenne muscular dystrophy.
机构信息
Infection and Epidemiology Department, Experimental Neuropathology Unit, Institut Pasteur, Paris, France.
Sorbonne Université, Assistance Publique - Hôpitaux de Paris, Department of Anesthesiology and Critical Care Medicine, Pitié-Salpêtrière Hospital, Paris, France.
出版信息
Muscle Nerve. 2019 Sep;60(3):328-335. doi: 10.1002/mus.26614. Epub 2019 Jul 16.
INTRODUCTION
No etiologic therapy is available for Duchenne muscular dystrophy (DMD), but mesenchymal stem cells were shown to be effective in preclinical models of DMD. The objective of this study is to investigate the effect of microfragmented fat extracted on a murine model of DMD.
METHODS
Fat tissue was extracted from healthy human participants and injected IM into DMD mice. Histological analysis, cytokines, and force measurement were performed up to 4 weeks after injection.
RESULTS
Duchenne muscular dystrophy mice injected with microfragmented fat exhibited an improved muscle phenotype (decreased necrosis and fibrosis), a decrease of inflammatory cytokines, and increased strength.
DISCUSSION
Administration of microfragmented fat in key muscles may improve muscular phenotype in patients with DMD. Muscle Nerve, 2019.
简介
目前尚无针对杜氏肌营养不良症（DMD）的病因疗法，但间充质干细胞在 DMD 的临床前模型中显示出有效性。本研究的目的是研究微碎片脂肪提取对 DMD 小鼠模型的影响。
方法
从健康的人类参与者中提取脂肪组织，并将其注射到 DMD 小鼠的肌肉中。在注射后 4 周内进行组织学分析、细胞因子和力量测量。
结果
注射微碎片脂肪的 DMD 小鼠表现出改善的肌肉表型（减少坏死和纤维化）、炎症细胞因子减少以及力量增加。
讨论
在关键肌肉中给予微碎片脂肪可能会改善 DMD 患者的肌肉表型。肌肉神经，2019 年。
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