Kurochkina L P, Komissarov A A, Kolomiĭtseva G Ia
Biokhimiia. 1987 Oct;52(10):1683-7.
A thymine-modified derivative of histone H3 formed as a result of thermal treatment of UV-irradiated (lambda = 254 nm) solution of deoxyribonucleoprotein from calf thymus at low ionic strength was isolated. The peptides obtained by tryptic hydrolysis of modified histone H3 were separated by high pressure liquid chromatography. The amino acid sequence of the peptide containing a lysine residue with covalently linked thymine was determined by the Edman method. It was found that Lys localized at the N-terminus of the histone H3 molecule interacts with DNA within the composition of the deoxyribonucleoprotein.
分离出了一种组蛋白H3的胸腺嘧啶修饰衍生物，它是由低离子强度下小牛胸腺脱氧核糖核蛋白的紫外线（λ = 254 nm）照射溶液经过热处理形成的。通过高压液相色谱法分离了经胰蛋白酶水解修饰组蛋白H3得到的肽段。采用埃德曼法测定了含有与胸腺嘧啶共价连接的赖氨酸残基的肽段的氨基酸序列。结果发现，位于组蛋白H3分子N端的赖氨酸在脱氧核糖核蛋白组成中与DNA相互作用。
