Department of Microbiology, Raiganj University, Raiganj, Uttar Dinajpur, 733134, India.
Bioinformatics Centre, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata 700 054, India.
Gene. 2019 Aug 30;711:143934. doi: 10.1016/j.gene.2019.06.024. Epub 2019 Jun 19.
Phytopathogenic fungi secrete a wide range of enzymes to penetrate and colonize host tissues. Of them protease activity is reported to increase disease aggressiveness in the plant. With the aim to explore the reason of the higher infection potential of proteases, we have compared several genomic and proteomic attributes among different hydrolytic enzymes coded by five pathogenic fungal species which are the potent infectious agents of plant. Categorizing the enzymes into four major groups, namely protease, lipase, amylase and cell-wall degraders, we observed that proteases are evolutionary more conserved, have higher expression levels, contain more hydrophobic buried residues, short linear motifs and post-translational modified (PTM) sites than the other three groups of enzymes. Again, comparing these features of protease between pathogenic and non-pathogenic Aspergillus sps, we have hypothesized that protein structural properties could play significant roles in imposing infection potency to the fungal proteases.
植物病原真菌分泌广泛的酶来穿透和定植宿主组织。据报道，其中蛋白酶活性会增加植物疾病的侵袭性。为了探究蛋白酶更高感染潜能的原因，我们比较了编码于五种病原真菌物种中的几种水解酶的基因组和蛋白质组特性，这些真菌物种是植物的强效传染性病原体。我们将这些酶分为四大类，即蛋白酶、脂肪酶、淀粉酶和细胞壁降解酶，结果观察到蛋白酶在进化上更为保守，表达水平更高，含有更多的疏水性埋藏残基、短线性基序和翻译后修饰（PTM）位点，比其他三类酶更多。此外，我们比较了病原性和非病原性 Aspergillus sps 之间蛋白酶的这些特性，我们假设蛋白质结构特性可能在赋予真菌蛋白酶感染能力方面发挥重要作用。
