大鼠体内生物素和生物胞素的肠道吸收

Intestinal absorption of biotin and biocytin in the rat.

作者信息

Dakshinamurti K, Chauhan J, Ebrahim H

机构信息

Department of Biochemistry, University of Manitoba, Winnipeg, Canada.

出版信息

Biosci Rep. 1987 Aug;7(8):667-73. doi: 10.1007/BF01127680.

DOI:10.1007/BF01127680

PMID:3122856

Abstract

The uptake of biotin and biocytin was investigated in rat intestine using the everted sac technique. It has been shown that at biotin and biocytin concentrations less than 40 and 50 nM respectively, absorption proceeds by a saturable process, whereas at higher concentrations uptake by passive diffusion predominates. Fractionation of solubilized brush border preparations indicates that biotinidase is the only protein which binds biotin in this preparation.

摘要

采用外翻肠囊技术研究了大鼠肠道对生物素和生物胞素的摄取。结果表明，当生物素和生物胞素浓度分别低于40 nM和50 nM时，吸收通过可饱和过程进行，而在较高浓度下，被动扩散摄取占主导。对溶解的刷状缘制剂进行分级分离表明，生物素酶是该制剂中唯一能结合生物素的蛋白质。