College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Infect Genet Evol. 2019 Oct;74:103932. doi: 10.1016/j.meegid.2019.103932. Epub 2019 Jun 20.
In avian species, the Reticuloendotheliosis virus (REV) causes severe immunosuppression and other symptoms, including avian dwarfing syndrome, and chronic tumors in lymphoid and other tissues. The pathogenesis of REV and its interaction with the host have yet to be fully elucidated with transcriptional studies on the changes in host gene expression after REV infection at the body level. In this study, the Spleen Necrosis Virus (SNV) was used to inoculate the one-day-old specific pathogen free (SPF) chicken to simulate congenital infection. We identified 1507 differentially expressed genes (DEGs) at 7, 14 and 21 dpi using Next Generation Sequencing (NGS) technology. Through the Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of these DEGs, it was found that DEGs were mainly involved in the categories of signal transduction, immune system and signaling molecules and interaction. Among them, Pattern recognition receptors (PRRs), chemokine, T cell receptor, JAK-STAT, TNF, and NF-kappa B signaling pathway, and the Hematopoietic cell lineage play an important role in the tumorigenic and immunosuppressive regulation of REV. In addition, a series of DEGs associated with inflammatory factors (CCL4, TNFRSF18, CDKN2), apoptosis (IRF1, PDCD1, WNT5A), innate immunity (TLR, MAD5, TRIM25), and adaptive immunity (LY6E, CD36, LAG3) were also discovered. We further verified 33 selected immune- relevant DEGs using quantitative RT-PCR (qRT-PCR). These findings provide new insights and research directions for revealing the pathogenesis of REV infection and the interaction between REV and the chicken immune system.
在禽类中，网状内皮组织增殖病病毒（REV）可导致严重的免疫抑制和其他症状，包括禽类侏儒综合征和淋巴样及其他组织的慢性肿瘤。REV 的发病机制及其与宿主的相互作用尚未通过对感染 REV 后宿主基因表达变化的转录研究得到充分阐明。在本研究中，我们使用脾脏坏死病毒（SNV）接种一日龄无特定病原体（SPF）鸡，以模拟先天性感染。我们使用下一代测序（NGS）技术在 7、14 和 21dpi 时鉴定了 1507 个差异表达基因（DEGs）。通过对这些 DEGs 的基因本体论（GO）富集和京都基因与基因组百科全书（KEGG）途径分析，发现 DEGs 主要涉及信号转导、免疫系统和信号分子和相互作用等类别。其中，模式识别受体（PRRs）、趋化因子、T 细胞受体、JAK-STAT、TNF 和 NF-kappa B 信号通路以及造血细胞谱系在 REV 的肿瘤发生和免疫抑制调节中发挥重要作用。此外，还发现了一系列与炎症因子（CCL4、TNFRSF18、CDKN2）、凋亡（IRF1、PDCD1、WNT5A）、先天免疫（TLR、MAD5、TRIM25）和适应性免疫（LY6E、CD36、LAG3）相关的 DEGs。我们进一步使用定量 RT-PCR（qRT-PCR）验证了 33 个选定的免疫相关 DEGs。这些发现为揭示 REV 感染的发病机制以及 REV 与鸡免疫系统的相互作用提供了新的见解和研究方向。
