Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University, Daizong ST, Tai'an, China.
Microb Pathog. 2012 Aug;53(2):57-63. doi: 10.1016/j.micpath.2012.04.001. Epub 2012 May 3.
Avian reticuloendotheliosis virus (REV) infection can induce a runting syndrome, immunosuppression, acute reticulum cell neoplasia and lymphomas in a variety of domestic and wild birds. To evaluate the pathogenicity and oncogenicity of REV-JX0927 that isolated from Chinese partridge, experimental inoculated day-old specific-pathogen-free (SPF) White Leghorn chickens were examined at regular intervals. The examination procedures included hematology, serology and histopathology; also including immunohistochemistry and apoptosis assay. Body weight, relative immune organs weight and apoptosis assay results revealed that the immunosuppression of infected birds is associated with apoptosis of lymphocytes in lymphoid tissues, especially in thymus induced by REV-JX0927. Hematology and apoptosis assay results showed that the 7th week of post-infection is a critical time point for lymphocytes to be transformed into tumor cells. Histopathology evidences demonstrated that REV-JX0927 induced reticuloendotheliosis at early stage (1 week), and lymphosarcomas at middle stage (after 7 weeks). In addition, squamous-cell carcinoma, adenocarcinoma and aneurysm were found in infected birds. Arteritis was associated with concentration of serum protein and fat. REV antigen expression was observed in infected birds through the experimental period. REV has high tropism for proventriculus, kidney, liver, lymphoid tissues, pancreas, lymphosarcoma cells and blood vessels. Data from this study showed that several new pathogenitic characters caused by REV-JX0927 were observed. It indicated that REV-JX0927 is a multipotential oncogenic retrovirus.
禽网状内皮组织增生病病毒（REV）感染可引起多种家禽和野生鸟类的矮小综合征、免疫抑制、急性网状内皮细胞肿瘤和淋巴瘤。为评估从雉鸡中分离到的 REV-JX0927 的致病性和致癌性，将其接种到 1 日龄无特定病原体（SPF）白来航鸡，定期进行检查。检查程序包括血液学、血清学和组织病理学；还包括免疫组织化学和凋亡检测。体重、相对免疫器官重量和凋亡检测结果表明，感染鸡的免疫抑制与淋巴组织中淋巴细胞的凋亡有关，特别是由 REV-JX0927 诱导的胸腺淋巴细胞凋亡。血液学和凋亡检测结果表明，感染后第 7 周是淋巴细胞转化为肿瘤细胞的关键时间点。组织病理学证据表明，REV-JX0927 早期（1 周）引起网状内皮细胞增多症，中期（7 周后）引起淋巴肉瘤。此外，感染鸡还发现了鳞状细胞癌、腺癌和动脉瘤。动脉炎与血清蛋白和脂肪浓度有关。在整个实验期间，通过免疫组织化学观察到感染鸡中存在 REV 抗原表达。REV 对鸡的前胃、肾脏、肝脏、淋巴组织、胰腺、淋巴肉瘤细胞和血管具有高亲嗜性。本研究的数据表明，观察到了由 REV-JX0927 引起的几种新的致病特征。这表明 REV-JX0927 是一种多潜能致癌逆转录病毒。
