Department of Pharmacology, Brain Repair Centre, Faculty of Medicine, Dalhousie University, 1348 Summer Street, Life Sciences Research Institute, North Tower, Halifax B3H 4R2, Canada.
Department of Pharmacology, Brain Repair Centre, Faculty of Medicine, Dalhousie University, 1348 Summer Street, Life Sciences Research Institute, North Tower, Halifax B3H 4R2, Canada; Department of Psychiatry, Brain Repair Centre, Faculty of Medicine, Dalhousie University, 1348 Summer Street, Life Sciences Research Institute, North Tower, Halifax B3H 4R2, Canada.
J Neuroimmunol. 2019 Sep 15;334:576995. doi: 10.1016/j.jneuroim.2019.576995. Epub 2019 Jun 15.
Experimental autoimmune encephalomyelitis (EAE) and lysophosphatidylcholine (LPC)-induced demyelination were combined to study remyelination in a pro-inflammatory context. Two groups of female C57BL/6 mice were subjected either to EAE (EAE mice) or injected with just complete Freund's adjuvant (CFA) and pertussis toxin (PTX) followed by bilateral LPC and phosphate buffered saline injections in the corpus callosum on day 7 (CFA controls). Relative to CFA controls, EAE accelerated remyelination and increased innate immune cell activation, lymphocyte infiltration and cytokine gene expression in the LPC lesions. However, compared to CFA mice, remyelination was reduced (day 14) suggesting this aggressive immune response also compromised myelin repair in EAE mice.
实验性自身免疫性脑脊髓炎（EAE）和溶血磷脂酰胆碱（LPC）诱导的脱髓鞘相结合，以研究促炎环境中的髓鞘再生。将两组雌性 C57BL/6 小鼠分别进行 EAE（EAE 小鼠）或仅用完全弗氏佐剂（CFA）和百日咳毒素（PTX）处理，然后在第 7 天在胼胝体中进行双侧 LPC 和磷酸盐缓冲盐水注射（CFA 对照组）。与 CFA 对照组相比，EAE 加速了髓鞘再生，并增加了 LPC 病变中固有免疫细胞的激活、淋巴细胞浸润和细胞因子基因表达。然而，与 CFA 小鼠相比，髓鞘再生减少（第 14 天），这表明这种侵袭性免疫反应也损害了 EAE 小鼠的髓鞘修复。
