Tríbulo Paula, Rivera Rocío Melissa, Ortega Obando Martha Sofia, Jannaman Elizabeth Ann, Hansen Peter J
Department of Animal Sciences, University of Florida, Gainesville, FL, USA.
Division of Animal Sciences, University of Missouri, Columbia, MO, USA.
Methods Mol Biol. 2019;2006:115-129. doi: 10.1007/978-1-4939-9566-0_8.
A protocol for production of bovine embryos from oocytes collected from ovaries obtained from an abattoir is described. The protocol includes methods for in vitro maturation of oocytes, capacitation of sperm, fertilization, and development of the resultant embryos to the blastocyst stage. The protocol can be easily modified to use oocytes collected by ultrasound-guided follicular aspiration.
本文描述了一种利用从屠宰场获取的卵巢中采集的卵母细胞生产牛胚胎的方案。该方案包括卵母细胞体外成熟、精子获能、受精以及将所得胚胎培养至囊胚阶段的方法。该方案可轻松修改，以使用通过超声引导卵泡抽吸采集的卵母细胞。
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