Berg Debra K, Thongphakdee Ampika
AgResearch, Ltd. Ruakura Research Centre, Hamilton, New Zealand.
Wildlife Reproductive Innovation Center, Research Department, Bureau of Conservation and Research, Zoological Park Organization under the Royal Patronage of H.M. the King, Bangkok, Thailand.
Methods Mol Biol. 2019;2006:191-207. doi: 10.1007/978-1-4939-9566-0_14.
In vitro embryo production of deer species has the potential to increase valuable traits for the agricultural sector, and from a conservation perspective, it is a propagation tool which can improve genetic diversity in small captive populations. In vitro embryo production is a multistep process consisting of oocyte maturation, fertilization, and embryo culture. These techniques provide the backbone for more advanced assisted reproductive technologies such as intracytoplasmic sperm injection (ICSI), somatic cell nuclear transfer (SCNT), a source of embryonic stem cells, and embryos for gene editing. In vitro-produced embryos are a readily available resource for comparative embryology studies and a functional assay to assess oocyte competence and evaluate in vitro embryo requirements during culture. A semidefined fertilization and culture medium system, deer synthetic oviduct fluid (DSOF), has been formulated based on deer oviduct fluid. Red deer calves (Cervus elaphus) and Thamin Eld's deer fawn (Rucervus eldii thamin) have been produced after the transfer of in vitro embryos (IVF and SCNT) grown in DSOF culture. Here we describe the in vitro method of maturation, fertilization, and embryo culture for deer species.
鹿类动物的体外胚胎生产有潜力为农业部门增加有价值的性状，并且从保护角度来看，它是一种繁殖工具，可提高小型圈养种群的遗传多样性。体外胚胎生产是一个多步骤过程，包括卵母细胞成熟、受精和胚胎培养。这些技术为更先进的辅助生殖技术提供了基础，如胞浆内单精子注射（ICSI）、体细胞核移植（SCNT）、胚胎干细胞来源以及用于基因编辑的胚胎。体外生产的胚胎是比较胚胎学研究的现成资源，也是评估卵母细胞能力和评估培养过程中体外胚胎需求的功能测定方法。一种半限定的受精和培养基系统，即鹿合成输卵管液（DSOF），已根据鹿输卵管液配制而成。在将在DSOF培养基中培养的体外胚胎（IVF和SCNT）移植后，已培育出红鹿幼崽（马鹿）和泽鹿幼崽（ Eld's鹿）。在这里，我们描述鹿类动物卵母细胞成熟、受精和胚胎培养的体外方法。
