Korzekwa Anna J, Kotlarczyk Angelika M, Szczepańska Agata A, Grzyb Martyna, Siergiej Alicja, Wocławek-Potocka Izabela
Department of Biodiversity Protection, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences (IAR&FR PAS), Tuwima 10 Str., 10-748 Olsztyn, Poland.
Department of Gamete and Embryo Biology, IAR&FR PAS, Tuwima 10 Str., 10-748 Olsztyn, Poland.
Animals (Basel). 2020 Jul 14;10(7):1190. doi: 10.3390/ani10071190.
The aim was to compare the blastocyst stages of red deer embryos in respect of in vitro fertilization (IVF) efficiency, morphology, apoptotic and proliferative abilities, and antioxidative potential according to the reproductive status of hinds. We used three experimental groups, including the ovaries collected post mortem on the 4th and 13th days of the estrous cycle and during pregnancy ( = 18). After oocyte maturation, frozen-thawed epididymal semen was used for IVF. Blastocyst quality, apoptotic potential by determining the mRNA expression of , , , , and placenta-specific 8 gene () and antioxidative potential of blastocysts were evaluated by determining the mRNA expression of , , and as well as the enzymatic activity of superoxide dismutase and reduced glutathione. The highest development rate of expanded blastocyst, mRNA expression of , , , and and mRNA expression and enzymatic activity of the antioxidative factors increased ( 0.05) in blastocysts developed from the oocytes collected on the 4th day, compared to those developed from the oocytes collected on the 13th day of the cycle and during pregnancy. Our study indicates that the 4th day of the estrous cycle is the most effective period for oocyte collection for IVF and embryo development in hinds, considering quality parameters and antioxidative potential of the blastocysts.
目的是根据母鹿的繁殖状态，比较马鹿胚胎的囊胚阶段在体外受精（IVF）效率、形态、凋亡和增殖能力以及抗氧化潜力方面的差异。我们使用了三个实验组，包括在发情周期的第4天和第13天以及妊娠期采集的卵巢（ = 18）。卵母细胞成熟后，使用冻融的附睾精液进行体外受精。通过测定 、 、 、 和胎盘特异性8基因（）的mRNA表达来评估囊胚质量、凋亡潜力，并通过测定 、 和 的mRNA表达以及超氧化物歧化酶和还原型谷胱甘肽的酶活性来评估囊胚的抗氧化潜力。与在发情周期第13天和妊娠期采集的卵母细胞发育而成的囊胚相比，从第4天采集的卵母细胞发育而成的囊胚中，扩张囊胚的最高发育率、 、 、 和 的mRNA表达以及抗氧化因子的mRNA表达和酶活性均有所增加（ 0.05）。我们的研究表明，考虑到囊胚的质量参数和抗氧化潜力，发情周期的第4天是母鹿体外受精和胚胎发育采集卵母细胞的最有效时期。
