Govindasamy Niraimathi, Bedzhov Ivan
Embryonic Self-Organization Research Group, Max Planck Institute for Molecular Biomedicine, Münster, Germany.
Methods Mol Biol. 2019;2006:373-382. doi: 10.1007/978-1-4939-9566-0_25.
Key developmental processes of cell fate decisions and morphogenetic transformations take place during the periimplantation and early postimplantation stages of mouse embryogenesis. However analysing these fundamental events relies on direct observations of cultured embryos, which are challenging to obtain. To address this challenge, here we provide a detailed protocol describing a workflow for isolating early implanted embryos, removing of redundant extraembryonic tissues and describing the culture conditions that support further embryo development in vitro.
细胞命运决定和形态发生转变的关键发育过程发生在小鼠胚胎发育的植入前期和植入后早期阶段。然而，分析这些基本事件依赖于对培养胚胎的直接观察，而获得这些胚胎具有挑战性。为应对这一挑战，我们在此提供一份详细的方案，描述了分离早期植入胚胎、去除多余的胚外组织以及描述支持胚胎在体外进一步发育的培养条件的工作流程。
