Kim Yung Su, Fan Rui, Kremer Ludmila, Kuempel-Rink Nannette, Mildner Karina, Zeuschner Dagmar, Hekking Liesbeth, Stehling Martin, Bedzhov Ivan
Embryonic Self-Organization Research Group, Max Planck Institute for Molecular Biomedicine, Röntgenstraße 20, 48149 Münster, Germany.
Transgenic Facility, Max Planck Institute for Molecular Biomedicine, Röntgenstraße 20, 48149 Münster, Germany.
Sci Adv. 2021 Mar 10;7(11). doi: 10.1126/sciadv.abe1640. Print 2021 Mar.
During the peri-implantation stages, the mouse embryo radically changes its appearance, transforming from a hollow-shaped blastocyst to an egg cylinder. At the same time, the epiblast gets reorganized from a simple ball of cells to a cup-shaped epithelial monolayer enclosing the proamniotic cavity. However, the cavity's function and mechanism of formation have so far been obscure. Through investigating the cavity formation, we found that in the epiblast, the process of lumenogenesis is driven by reorganization of intercellular adhesion, vectoral fluid transport, and mitotic paracellular water influx from the blastocoel into the emerging proamniotic cavity. By experimentally blocking lumenogenesis, we found that the proamniotic cavity functions as a hub for communication between the early lineages, enabling proper growth and patterning of the postimplantation embryo.
在植入前阶段，小鼠胚胎的外观发生了根本性变化，从空心的囊胚转变为卵圆柱状。与此同时，上胚层从一个简单的细胞球重组为包围羊膜腔的杯状上皮单层。然而，该腔的功能和形成机制迄今仍不清楚。通过研究腔的形成，我们发现在上胚层中，管腔发生过程是由细胞间粘附的重组、矢量液体运输以及有丝分裂期间细胞旁水从囊胚腔流入新形成的羊膜腔所驱动的。通过实验阻断管腔发生，我们发现羊膜腔作为早期谱系之间通讯的枢纽，使植入后胚胎能够正常生长和形成模式。
