University of Nottingham, Sutton Bonington, UK.
Newcastle University, Newcastle Upon Tyne, UK.
Parasite Immunol. 2019 Sep;41(9):e12660. doi: 10.1111/pim.12660. Epub 2019 Jul 24.
Very little has been reported comparing resistance to coccidiosis in fast or slow growing broilers, the latter of which are becoming more prevalent in the broiler industry. We examined mRNA expression in the intestines of fast and slow growing broilers following Eimeria infection. We show that by day 13 post-infection (d pi) with 2500 or 7000 oocysts of Eimeria maxima, slower-growing (Ranger Classic) broilers significantly (P < 0.01) upregulated expression of proinflammatory cyclooxygenase genes (LTB4DH, PTSG1 and PTSG2) above that detected in fast growing (Ross 308) broilers. Expression of CD8α mRNA was downregulated in Ross 308 at day 6d pi with either 2500 or 7000 oocysts of E maxima (P < 0.05), compared to uninfected controls, but was not differentially expressed in Ranger Classic. CD4 genes were not differentially expressed in either chicken line infected with either infectious oocyst dose at d6 pi, compared to uninfected controls. However, at d13 pi, CD4 expression was significantly upregulated in both chicken lines infected with either infectious oocyst dose, compared to uninfected controls (P < 0.05) but this was significantly greater in Ranger Classic broilers compared to Ross 308 (P < 0.05). At d13 pi, expression of CD3 chains (required for T lymphocyte activation) was significantly increased in Ranger Classic compared to Ross 308, infected with either oocyst dose (P < 0.05-0.01). Expression of IL-2 and IL-15 mRNA, required for T lymphocyte proliferation was also significantly upregulated, or maintained longer, in Ranger Classic broilers compared to Ross 308. These differences in immune response to E maxima corresponded with a reduction in E maxima genome detected in the intestines of Ranger Classic compared to Ross 308.
在快速生长和缓慢生长的肉鸡中，对抗球虫病的抗性差异很少被报道，而后者在肉鸡行业中越来越普遍。我们研究了感染艾美耳球虫后快速生长和缓慢生长肉鸡的肠道中 mRNA 的表达情况。结果表明，在感染艾美耳球虫 13 天后（dpi），用 2500 或 7000 个艾美耳球虫卵囊感染，生长较慢（Ranger Classic）的肉鸡显著（P < 0.01）上调了促炎环氧化酶基因（LTB4DH、PTSG1 和 PTSG2）的表达，高于快速生长（Ross 308）肉鸡。与未感染对照组相比，在感染 2500 或 7000 个艾美耳球虫卵囊的 Ross 308 肉鸡中，CD8α mRNA 的表达在第 6 天 dpi 时下调（P < 0.05），但在 Ranger Classic 中没有差异表达。在感染两种传染性卵囊剂量的肉鸡中，CD4 基因在任何一种鸡线中都没有差异表达，与未感染对照组相比。然而，在第 13 天 dpi 时，两种鸡线感染任意传染性卵囊剂量后，CD4 的表达均显著上调，与未感染对照组相比（P < 0.05），但 Ranger Classic 肉鸡中的表达明显高于 Ross 308（P < 0.05）。在第 13 天 dpi 时，与 Ross 308 相比，Ranger Classic 中 CD3 链（T 淋巴细胞激活所需）的表达显著增加，感染任意卵囊剂量（P < 0.05-0.01）。T 淋巴细胞增殖所需的 IL-2 和 IL-15 mRNA 的表达也显著上调，或在 Ranger Classic 肉鸡中维持时间更长，与 Ross 308 相比。与 Ross 308 相比，Ranger Classic 肉鸡中对 E 最大的免疫反应的这些差异与在 Ranger Classic 肉鸡中检测到的 E 最大基因组的减少相对应。
