Department of Pharmacognosy Faculty of pharmacy, Cairo University, Cairo, Egypt.
Department of Timber Trees Research, Horticultural Research Institute (ARC), Giza, Egypt.
Nat Prod Res. 2020 Nov;34(21):3125-3129. doi: 10.1080/14786419.2019.1607334. Epub 2019 Jun 23.
The aim of our study is to estimate the hepatoprotective effects of the ethanolic extract of the leaves of by determination of liver biomarkers (ALT, AST, total bilirubin and albumin in serum) and by histopathological examinations using thioacetamide-induced (TAA) liver injury model. Concurrent administration of ethanolic extracts of leaves improved the alterations in liver morphology where it was a potent protector of the liver. The potential of L-phenylalanine and silver nitrate as chemical elicitors as well as UV radiation as a physical elicitor on flavonoid production in callus culture of were emphasized. Murashige and Skoog's medium fortified with phenylalanine and silver nitrate enhanced the production of flavonoids and phenolics. HPLC analysis was performed for qualitative and quantitative estimation of some flavonoid compounds in the produced calli in comparison with the mother plant. This finding highlights the potential use of in the treatment of liver dysfunction.
本研究旨在通过测定血清中肝生物标志物（ALT、AST、总胆红素和白蛋白）以及通过硫代乙酰胺诱导（TAA）肝损伤模型的组织病理学检查，来评估 的叶乙醇提取物的保肝作用。同时给予 的叶乙醇提取物可改善肝形态的改变，表明其对肝脏有很强的保护作用。强调了 L-苯丙氨酸和硝酸银作为化学诱导剂以及 UV 辐射作为物理诱导剂对 愈伤组织中类黄酮产生的影响。添加苯丙氨酸和硝酸银的 Murashige 和 Skoog 培养基可增强类黄酮和酚类物质的产生。通过 HPLC 分析对产生的愈伤组织中的一些类黄酮化合物进行定性和定量估计，并与母株进行比较。这一发现突出了 在治疗肝功能障碍方面的潜在用途。
