Division of Pharmacognosy, Pharmacognosy and Phytotherapy Research Laboratory, Department of Pharmaceutical Technology, Faculty of Engineering and Technology, Jadavpur University, Kolkata, India.
Ann Hepatol. 2011 Jan-Mar;10(1):63-72.
The aim of this study was to investigate prophylactic and curative effect of polyphenolic extract of Ichnocarpus frutescense against carbon tetrachloride and tamoxifen induced hepatotoxicity in rats. Carbon tetrachloride and tamoxifen caused liver damage in rats manifested by significant rise in serum enzymes levels. Models of carbon tetrachloride and tamoxifen intoxication elicited significant declines in the reduced glutathione concomitant with significant elevations in malondialdehyde levels. The oral administration of polyphenolic extract to carbon tetrachloride and tamoxifen intoxicated ats, produced significant increments in the reduced glutathione concomitant with significant decrements in malondialdehyde and liver transaminases levels. Prophylactic and curative treatments with the polyphenolic extract generally resulted in a relatively good protection against both carbon tetrachloride and tamoxifen intoxicated rats. The histopathological changes of liver sections showed an improved histological appearance. The extract inhibits CYP monoxygenases aminopyrine-N-demethylase and aniline hydroxylase, suggesting a plausible hepatoprotective mechanism. However prophylactic treatment with the polyphenolic extract exhibited a higher activity compared to curative treatment. The normalization of phenobarbitone induced sleeping time suggests the restoration of liver CYP enzymes. The study shows that hepatoprotective activity of polyphenol extract is by regulating the levels of hepatic microsomal drug metabolising enzymes. These results supported the use of this plant for the treatment of hepatitis in oriental traditional medicine.
本研究旨在探讨榼藤子多酚提取物对四氯化碳和他莫昔芬诱导的大鼠肝毒性的预防和治疗作用。四氯化碳和他莫昔芬可导致大鼠肝损伤，表现为血清酶水平显著升高。四氯化碳和他莫昔芬中毒模型引起还原型谷胱甘肽水平显著下降，同时丙二醛水平显著升高。多酚提取物对四氯化碳和他莫昔芬中毒大鼠的口服给药可显著增加还原型谷胱甘肽水平，同时显著降低丙二醛和肝转氨酶水平。预防性和治疗性使用多酚提取物通常可对两种中毒大鼠提供相对较好的保护作用。肝切片的组织病理学变化显示出改善的组织学外观。提取物抑制 CYP 单加氧酶氨基比林-N-脱甲基酶和苯胺羟化酶，提示存在合理的保肝机制。然而，与治疗性治疗相比，预防性治疗显示出更高的活性。苯巴比妥诱导的睡眠时间的正常化表明肝 CYP 酶的恢复。该研究表明，多酚提取物的保肝活性是通过调节肝微粒体药物代谢酶的水平来实现的。这些结果支持将这种植物用于东方传统医学中治疗肝炎。
