Shaw R W, Ndukwe G, Imoedemhe D, Burford G, Chan R
Academic Department of Obstetrics & Gynaecology, Royal Free Hospital, London, United Kingdom.
Clin Reprod Fertil. 1987 Jun;5(3):141-51.
Administration of pulsatile LHRH (14.4 microgram/pulse per 90 min) from day 5 to 9, or day 7 to 11 of the cycle following clomiphene pretreatment on days 2-6 induced multiple follicular growth for oocyte recovery for in vitro fertilisation. Administration of LHRH without clomiphene pretreatment failed to induce multiple follicular development. The same group of patients treated with clomiphene alone had fewer numbers of mature follicles, fewer oocytes recovered and less pre-embryos for transfer than when treated with clomiphene and LHRH in combination. Peak gonadotrophin release following commencement of LHRH was seen within the first 24 h of administration with a gradual fall in both LH and FSH toward baseline pretreatment values by the fourth day of administration. A discernible LH increment to LHRH was still present on the fourth day of LHRH treatment, but in many individuals no FSH increment was observed. The preovulatory oocytes recovered were predominantly mature with fertilisation rates of 70% and 85.7% for the two regimens. No pregnancy occurred in the six patients who underwent pre-embryo transfer.
在克罗米芬于周期第2 - 6天进行预处理后，于周期第5至9天或第7至11天给予脉冲式促黄体生成素释放激素（LHRH，14.4微克/脉冲，每90分钟一次），可诱导多个卵泡生长，以便回收卵母细胞用于体外受精。未进行克罗米芬预处理而给予LHRH未能诱导多个卵泡发育。同一组患者单独使用克罗米芬治疗时，与联合使用克罗米芬和LHRH治疗相比，成熟卵泡数量更少，回收的卵母细胞更少，可供移植的胚胎前体也更少。开始给予LHRH后，促性腺激素释放峰值在给药的头24小时内出现，到给药第4天时，促黄体生成素（LH）和促卵泡生成素（FSH）均逐渐降至预处理时的基线值。在LHRH治疗第4天时，仍可观察到对LHRH有明显的LH升高，但在许多个体中未观察到FSH升高。回收的排卵前卵母细胞大多成熟，两种方案的受精率分别为70%和85.7%。接受胚胎前体移植的6名患者均未怀孕。
