Şahin Davut, Çiçek Bahattin, Akpolat İlkser, Şişman Gürhan, Tekkeşin Nilgün
Department of Pathology-Cytopathology, Acıbadem Health Group, İstanbul, Turkey.
Department of Gastroenterology, Acıbadem Mehmet Ali Aydınlar University, İstanbul, Turkey.
Turk J Gastroenterol. 2019 Oct;30(10):903-909. doi: 10.5152/tjg.2019.19006.
BACKGROUND/AIMS: The aim of the present study was to investigate whether pancreas cyst fluids should be divided into two for cytological diagnosis and biochemical tests.
The present study was conducted with fluids aspirated from 12 pancreas cysts. The fluids were divided into two and sent to the cytopathology (fluid 1) and biochemistry (fluid 2) laboratories. Fluid 1 was centrifuged at the cytopathology laboratory. Cytology slides were prepared from the deposit, and the supernatant was sent to the biochemistry laboratory. Fluid 2 was centrifuged at the biochemistry laboratory, and amylase, carcinoembryonic antigen, and cancer antigen 19.9 levels were determined in the supernatant. These procedures were repeated for fluid 1 from the cytopathology laboratory. The remaining fluid 2 was sent to the cytopathology laboratory. Fluid 1-like slides were prepared from fluid 2 in the cytopathology laboratory. Cytological diagnoses of fluid 1 and fluid 2 were compared, and the Pearson correlation coefficient for biochemical test results was identified.
92% of fluid 1 and 50% of fluid 2 were diagnostic. Biochemical test results of fluid 1 and fluid 2 were similar, and the Pearson correlation coefficient was high.
Our results showed that pancreatic cyst fluids did not need to be divided into two for cytological diagnosis and biochemical tests. Following centrifugation of the whole fluid at the cytopathology laboratory, the deposit and the supernatant can be used for cytological diagnosis and for biochemical tests, respectively. With this protocol, the sensitivity of cytological diagnoses and biochemical tests of pancreatic cyst fluids may increase.
背景/目的：本研究的目的是调查胰腺囊肿液是否应分为两份分别进行细胞学诊断和生化检测。
本研究使用从12个胰腺囊肿中抽取的液体进行。将液体分为两份，分别送至细胞病理学实验室（液体1）和生化实验室（液体2）。液体1在细胞病理学实验室进行离心。从沉淀物制备细胞学玻片，上清液送至生化实验室。液体2在生化实验室进行离心，并测定上清液中的淀粉酶、癌胚抗原和癌抗原19.9水平。对细胞病理学实验室的液体1重复这些操作。剩余的液体2送至细胞病理学实验室。在细胞病理学实验室从液体2制备类似液体1的玻片。比较液体1和液体2的细胞学诊断结果，并确定生化检测结果的Pearson相关系数。
液体1的诊断率为92%，液体2的诊断率为50%。液体1和液体2的生化检测结果相似，Pearson相关系数较高。
我们的结果表明，胰腺囊肿液无需分为两份进行细胞学诊断和生化检测。在细胞病理学实验室对全液进行离心后，沉淀物和上清液可分别用于细胞学诊断和生化检测。采用此方案，胰腺囊肿液细胞学诊断和生化检测的敏感性可能会提高。
