α-干扰素和γ-干扰素对单核细胞和内皮细胞中凝血活酶反应的不同作用。
Differential effect of alpha-interferon and gamma-interferon on thromboplastin response in monocytes and endothelial cells.
作者信息
Carlsen E, Stinessen M B, Prydz H
机构信息
Research Institute for Internal Medicine, University of Oslo, Norway.
出版信息
Clin Exp Immunol. 1987 Nov;70(2):471-8.
Recombinant gamma-interferon (r gamma-IFN) has contrasting effects on thromboplastin (TPL) synthesis induced in monocytes (M) and endothelial cells by bacterial lipopolysaccharide (LPS), phorbol ester (TPA), and phytohaemagglutinin (PHA). In human umbilical vein endothelial cells (HUVEC) the induced thromboplastin response was significantly augmented by r gamma-IFN whereas the monocyte response was inhibited. Recombinant alpha-interferon (r alpha-IFN) had no effect on thromboplastin induction in endothelial cells but had a significant inhibitory effect on the TPL response in monocytes when LPS or LPS and cyclosporin A (CS) were used as inducing agents. Cyclosporin A, previously shown to enhance thromboplastin synthesis induced in monocytes, also contributes to a higher level of thromboplastin activity in endothelial cells. Its effect on monocytes was in most cases fully inhibited by r gamma-IFN (and also by r alpha-IFN when tested). gamma-Interferon and alpha-interferon alone had a weak stimulatory effect or none on thromboplastin synthesis in both cell types.
重组γ干扰素（rγ-IFN）对细菌脂多糖（LPS）、佛波酯（TPA）和植物血凝素（PHA）诱导单核细胞（M）和内皮细胞合成凝血活酶（TPL）具有相反的作用。在人脐静脉内皮细胞（HUVEC）中，rγ-IFN显著增强诱导的凝血活酶反应，而单核细胞反应则受到抑制。重组α干扰素（rα-IFN）对内皮细胞中凝血活酶的诱导没有影响，但当使用LPS或LPS与环孢素A（CS）作为诱导剂时，对单核细胞中的TPL反应具有显著的抑制作用。环孢素A先前已被证明可增强单核细胞中诱导的凝血活酶合成，它也有助于提高内皮细胞中凝血活酶的活性水平。在大多数情况下，其对单核细胞的作用被rγ-IFN（以及测试时的rα-IFN）完全抑制。单独的γ干扰素和α干扰素对两种细胞类型中的凝血活酶合成具有微弱的刺激作用或无作用。
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