Key Laboratory of Anti-Inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Center of Anti-Inflammatory and Immune Medicine, Institute of Clinical Pharmacology, Anhui Medical University, Hefei, China.
Department of Neurosurgery, First Affiliated Hospital of Anhui Medical University, Hefei, China.
Front Immunol. 2019 May 24;10:1146. doi: 10.3389/fimmu.2019.01146. eCollection 2019.
The ontogeny of macrophages in most organ/tissues in human body has been proven. Due to the limited number and inaccessibility of synovial macrophages (SM), the origin of SM has not been fully illuminated. The objective of this study was designed to investigate the ontogeny of SM and to evaluate the role of SM from different origins in arthritis. Two origins of SM, embryonic SM (ESM) and bone marrow SM (BMSM) were identified in Cx3cr1-EGFP mice, CCR2 mice and bone marrow (BM) chimera model by using a stringent sorting strategy. The cellular features, including dynamic total cell number, proliferation, phagocytosis and expressions of pro-inflammatory and anti-inflammatory genes, of ESM and BMSM were compared. In addition, ESM and BMSM showed different expression patterns in Rheumatoid Arthritis (RA) patients' synovium and during the developmental process of collagen-induced arthritis (CIA) mice. Taken together, these results demonstrated that the SM at least has two origins, ESM and BMSM. The different cellular property and dynamic expression patterns in RA patients/CIA mice highlight the notion that ESM and BMSM might play different role in arthritis.
已经证明，人体大多数器官/组织中的巨噬细胞的发生发展过程是存在的。由于滑膜巨噬细胞（SM）数量有限且难以获取，因此其起源尚未完全阐明。本研究旨在探究 SM 的发生发展，并评估来自不同起源的 SM 在关节炎中的作用。通过严格的分选策略，利用 Cx3cr1-EGFP 小鼠、CCR2 小鼠和骨髓嵌合体模型，确定了 SM 的两个来源，即胚胎期 SM（ESM）和骨髓 SM（BMSM）。比较了 ESM 和 BMSM 的细胞特征，包括总细胞数、增殖、吞噬作用以及促炎和抗炎基因的表达的动态变化。此外，在类风湿关节炎（RA）患者的滑膜组织和胶原诱导关节炎（CIA）小鼠的发育过程中，ESM 和 BMSM 表现出不同的表达模式。综上所述，这些结果表明 SM 至少有两个来源，即 ESM 和 BMSM。在 RA 患者/CIA 小鼠中，不同的细胞特性和动态表达模式提示 ESM 和 BMSM 可能在关节炎中发挥不同的作用。
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