Department of Oncological Sciences and Tisch Cancer Institute, Critical Care and Sleep Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.
Immunity. 2013 Apr 18;38(4):792-804. doi: 10.1016/j.immuni.2013.04.004.
Despite accumulating evidence suggesting local self-maintenance of tissue macrophages in the steady state, the dogma remains that tissue macrophages derive from monocytes. Using parabiosis and fate-mapping approaches, we confirmed that monocytes do not show significant contribution to tissue macrophages in the steady state. Similarly, we found that after depletion of lung macrophages, the majority of repopulation occurred by stochastic cellular proliferation in situ in a macrophage colony-stimulating factor (M-Csf)- and granulocyte macrophage (GM)-CSF-dependent manner but independently of interleukin-4. We also found that after bone marrow transplantation, host macrophages retained the capacity to expand when the development of donor macrophages was compromised. Expansion of host macrophages was functional and prevented the development of alveolar proteinosis in mice transplanted with GM-Csf-receptor-deficient progenitors. Collectively, these results indicate that tissue-resident macrophages and circulating monocytes should be classified as mononuclear phagocyte lineages that are independently maintained in the steady state.
尽管有越来越多的证据表明组织巨噬细胞在稳态下可以自我维持，但传统观点仍然认为组织巨噬细胞来源于单核细胞。通过联体共生和谱系示踪方法，我们证实单核细胞在稳态下对组织巨噬细胞的贡献不大。同样，我们发现，在耗尽肺巨噬细胞后，大多数再增殖是通过随机细胞增殖发生的，这是一种依赖于巨噬细胞集落刺激因子（M-CSF）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）的方式，但不依赖于白细胞介素-4。我们还发现，骨髓移植后，当供体巨噬细胞的发育受损时，宿主巨噬细胞仍有扩张的能力。宿主巨噬细胞的扩张是功能性的，可以防止接受 GM-CSF 受体缺陷祖细胞移植的小鼠发生肺泡蛋白沉积症。总的来说，这些结果表明，组织驻留巨噬细胞和循环单核细胞应被归类为单核吞噬细胞谱系，它们在稳态下是独立维持的。
