Tønnesen E, Christensen N J, Brinkløv M M
Department of Clinical Immunology, Odense University Hospital, Denmark.
Eur J Clin Invest. 1987 Dec;17(6):497-503. doi: 10.1111/j.1365-2362.1987.tb01148.x.
The effects of cortisol and adrenaline on natural killer (NK) cell activity and the distribution of circulating lymphocyte subpopulations were studied in twenty volunteers, using a continuous intravenous infusion pattern to simulate some of the hormonal changes induced by major surgery. The participants were allocated to receive either cortisol for 5 h, adrenaline for 1 h, cortisol for 5 h with simultaneous adrenaline during the last hour, or placebo for 5 h. Cortisol induced leucocytosis, neutrophilia, and lymphopenia with marked reduction in the number of T-lymphocyte subsets (OKT3+, OKT4+, and OKT8+ cells). No changes were induced in the activity or number of NK (Leu 11+) cells. Adrenaline produced an instantaneous increase in NK-cell activity accompanied by a selective increase in circulating NK cells. Significant leucocytosis, lymphocytosis and neutrophilia occurred. All measurements returned to preinfusion levels within 15 min after completing infusion. The effects of simultaneous infusion of cortisol and adrenaline were equal to the additive response to the hormones administered separately, except for the leucocytosis, which clearly exceeded this. In the placebo group all measurements remained unchanged. The results confirm the role of adrenaline as a potent stimulator/inducer of NK-cell activity. Adrenaline may be responsible for the increase in NK-cell activity during anaesthesia and major surgery.
在20名志愿者中研究了皮质醇和肾上腺素对自然杀伤（NK）细胞活性及循环淋巴细胞亚群分布的影响，采用持续静脉输注模式来模拟大手术引起的一些激素变化。参与者被分配接受5小时的皮质醇、1小时的肾上腺素、在最后1小时同时输注5小时皮质醇和肾上腺素，或5小时的安慰剂。皮质醇诱导白细胞增多、中性粒细胞增多和淋巴细胞减少，T淋巴细胞亚群（OKT3 +、OKT4 +和OKT8 +细胞）数量显著减少。NK（Leu 11 +）细胞的活性或数量未发生变化。肾上腺素使NK细胞活性瞬间增加，同时循环NK细胞选择性增加。出现显著的白细胞增多、淋巴细胞增多和中性粒细胞增多。输注结束后15分钟内所有测量值均恢复到输注前水平。同时输注皮质醇和肾上腺素的效果等于分别给予这两种激素的相加反应，但白细胞增多明显超过此值。在安慰剂组中，所有测量值均保持不变。结果证实了肾上腺素作为NK细胞活性的有效刺激物/诱导物的作用。肾上腺素可能是麻醉和大手术期间NK细胞活性增加的原因。
