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十二烷基硫酸钠-聚丙烯酰胺凝胶电泳在白喉棒状杆菌流行病学研究中的应用

The use of SDS-polyacrylamide gel electrophoresis in epidemiological studies of Corynebacterium diphtheriae.

作者信息

Hallas G

机构信息

Division of Hospital Infection, Central Public Health Laboratory, London, UK.

出版信息

Epidemiol Infect. 1988 Feb;100(1):83-90. doi: 10.1017/s0950268800065584.

DOI:10.1017/s0950268800065584
PMID:3123261
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2249200/
Abstract

Polyacrylamide gel electrophoresis of cell proteins was investigated as a possible typing method for Corynebacterium diphtheriae. A method was developed using stock strains which were representatives of the five gravis serotypes described by Robinson & Peeney (1936). This technique was then applied to recent isolates sent to our laboratory for identification.

摘要

对白喉棒状杆菌的细胞蛋白进行聚丙烯酰胺凝胶电泳研究，作为一种可能的分型方法。利用罗宾逊和皮尼（1936年）描述的五种重型血清型的代表菌株开发了一种方法。然后将该技术应用于送至我们实验室进行鉴定的近期分离株。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d62/2249200/87a2ea6b3d54/epidinfect00007-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d62/2249200/d64a75866774/epidinfect00007-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d62/2249200/a83365f766ad/epidinfect00007-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d62/2249200/504a0165b5c1/epidinfect00007-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d62/2249200/87a2ea6b3d54/epidinfect00007-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d62/2249200/d64a75866774/epidinfect00007-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d62/2249200/a83365f766ad/epidinfect00007-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d62/2249200/504a0165b5c1/epidinfect00007-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d62/2249200/87a2ea6b3d54/epidinfect00007-0090-a.jpg

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本文引用的文献

1
Classification of Corynebacterium species from axillary skin by numerical analysis of electrophoretic protein patterns.通过电泳蛋白质图谱的数值分析对腋窝皮肤棒状杆菌进行分类。
J Med Microbiol. 1982 Nov;15(4):485-92. doi: 10.1099/00222615-15-4-485.
2
Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis.通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析鼠伤寒沙门氏菌中的脂多糖异质性。
Eur J Biochem. 1980;107(1):137-43. doi: 10.1111/j.1432-1033.1980.tb04634.x.
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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
4
Polyacrylamide gel electrophoresis of Corynebacterium diphtheriae: a possible epidemiological aid.白喉棒状杆菌的聚丙烯酰胺凝胶电泳：一种可能有助于流行病学研究的方法。
Appl Microbiol. 1971 Nov;22(5):885-90. doi: 10.1128/am.22.5.885-890.1971.
5
Diphthericin types, bacteriophage types and serotypes of Corynebacterium diphtheriae strains isolated in Australia.在澳大利亚分离出的白喉棒状杆菌菌株的白喉菌素类型、噬菌体类型和血清型
J Hyg (Lond). 1973 Dec;71(4):679-89. doi: 10.1017/s0022172400022944.
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Characterization of Corynebacterium group JK by whole-cell protein patterns.通过全细胞蛋白质图谱对JK棒状杆菌属进行鉴定
J Gen Microbiol. 1986 Jul;132(7):1911-5. doi: 10.1099/00221287-132-7-1911.
7
Commercial strip test for reduction of nitrate by bacteria.用于检测细菌还原硝酸盐的商业试纸条检测。
J Clin Pathol. 1986 Jan;39(1):118. doi: 10.1136/jcp.39.1.118-a.